Stochastic Microsensors Based on Carbon Nanotubes for Molecular Recognition of the Isocitrate Dehydrogenases 1 and 2

Nanomaterials

Sorin Sebastian Gheorghe¹  Catalina Cioates Negut²  Raluca-Ioana Stefan-van Staden²  Paula Sfirloaga³ 

[1] Faculty of Applied Chemistry and Material Science, Politehnica University of Bucharest, 060021 Bucharest, Romania;Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei St., 060021 Bucharest, Romania;National Institute of Research for Electrochemistry and Condensed Matter, Dr. Aurel Paunescu Podeanu 144, 300569 Timisoara, Romania;

关键词: stochastic microsensors;    isocitrate dehydrogenase 1;    isocitrate dehydrogenase 2;    carbon nanotubes;   

DOI  :  10.3390/nano12030460

来源: DOAJ

【 摘 要 】

Two three-dimensional (3D) stochastic microsensors based on immobilization of protoporphyrin IX (PIX) in single-walled carbon nanotubes (SWCNT) and multi-walled carbon nanotubes (MWCNT) decorated with copper (Cu) and gold (Au) nanoparticles were designed and used for the molecular recognition of isocitrate dehydrogenase 1 (IDH1) and isocitrate dehydrogenase 2 (IDH2) in biological samples (brain tumor tissues, whole blood). The linear concentration ranges obtained for the molecular recognition and quantification of IDH1 and IDH2 were: IDH1 (1 × 10⁻⁵–1 × 10² ng mL⁻¹) and IDH2 (5 × 10⁻⁸ − 5 × 10² ng mL⁻¹). The limits of quantification obtained using the proposed microsensors were: 10 fg mL^–1 for IDH1 and 5 × 10⁻³ fg mL⁻¹ for IDH2. The highest sensitivities were obtained for the microsensor based on MWCNT. High recoveries versus enzyme-linked immunosorbent assay (ELISA) standard method were recorded for the assays of IDH1 and IDH2, all values being higher than 99.00%, with relative standard deviations (RSD) lower than 0.10%.

【 授权许可】

Unknown