Determination of Antioxidant, Antimicrobial Activity, Heavy Metals and Elements Content of Seaweed Extracts

Plants

Michal Miškeje¹  Petra Borotová¹  Maciej Kluz²  Lucia Galovičová³  Natália Čmiková³  Miroslava Kačániová³ 

[1] AgroBioTech Research Centre, Slovak University of Agriculture, Tr. A. Hlinku 2, 94976 Nitra, Slovakia;Department of Bioenergy, Food Technology and Microbiology, Institute of Food Technology and Nutrition, University of Rzeszow, 4 Zelwerowicza St, 35601 Rzeszow, Poland;Institute of Horticulture, Faculty of Horticulture, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia;

关键词: seaweed;    algae;    antimicrobial activity;    antioxidant activity;    heavy metals;    PCA;   

DOI  :  10.3390/plants11111493

来源: DOAJ

【 摘 要 】

The aim of the research was to determine the antioxidant and antimicrobial activity, determination of chemical elements and heavy metals in seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki. Antioxidant activity was determined by DPPH method and the activity ranged from 0.00 to 2641.34 TEAC. The highest antioxidant activity was observed in kombu (2641.34 TEAC) and arame (2457.5 TEAC). Antimicrobial activity was analyzed by disk diffusion method and MIC method. Three G⁺ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis), three G^- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica), and four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans) were used as model organisms. The size of inhibition zones ranged from 0.00 to 8.67 mm. The minimum inhibitory concentrations of the selected seaweeds ranged from MIC₅₀ 98.46 (MIC₉₀ 100.25) to MIC₅₀ 3.43 µL/mL (MIC₉₀ 5.26 µL/mL). The content of selected elements was determined in seaweed samples by ICP-OES. The chemical composition of the algae showed differences between species and the presence of heavy metals. Arsenic, cadmium, and aluminum were confirmed. All seaweed samples contained arsenic ranging from 6.6546 to 76.48 mg/kg. Further investigation of seaweeds is needed to identify the active substances present in the algae that are responsible for antioxidant and antimicrobial activity. This study was carried out to evaluate the antimicrobial and antioxidant activity of extracts from five commonly consumed seaweeds for their ability to inhibit selected microorganisms and to determine the health risk due to heavy metals content. Our study contributes to the evidence that seaweeds have antimicrobial and antioxidant activity and seaweed extracts have for pharmacological applications.

【 授权许可】

Unknown