CD4+FOXP3+ T Cells in Rheumatoid Arthritis Bone Marrow Are Partially Impaired

Cells

Monika Prochorec-Sobieszek¹  Magdalena Massalska²  Magdalena Plebanczyk²  Urszula Skalska²  Anna Radzikowska²  Weronika Kurowska²  Ewa Kontny²  Wlodzimierz Maslinski²  Ewa Kuca-Warnawin²  Hans-Jürgen Gober³  Pawel Maldyk⁴ 

[1] Department of Pathology, National Institute of Geriatrics, Rheumatology, and Rehabilitation, 02-637 Warsaw, Poland;Department of Pathophysiology and Immunology, National Institute of Geriatrics, Rheumatology, and Rehabilitation, 02-637 Warsaw, Poland;Department of Pharmacy, Kepler University Hospital, 4020 Linz, Austria;Department of Rheumoorthopaedic Surgery, National Institute of Geriatrics, Rheumatology, and Rehabilitation, 02-637 Warsaw, Poland;

关键词: bone marrow;    cd4⁺foxp3⁺;    treg cells;    rheumatoid arthritis;    immunosuppression;   

DOI  :  10.3390/cells9030549

来源: DOAJ

【 摘 要 】

There is evolving evidence that dysregulation of immune homeostasis in the bone marrow (BM) adjacent to the inflamed joints is involved in the pathogenesis of. In this study, we are addressing the phenotype and function of regulatory T cells (Tregs) residing in the BM of patients with rheumatoid arthritis (RA) and osteoarthritis (OA). BM and peripheral blood samples were obtained from RA and OA patients undergoing hip replacement surgery. The number and phenotype of Tregs were analyzed by flow cytometry and immunohistochemistry. The function of Tregs was investigated ex vivo, addressing their suppressive activity on effector T cells. [³H]-Thymidine incorporation assay and specific enzyme-linked immunosorbent assay were used for quantification of cell proliferation and pro-inflammatory (TNF, IFN-γ) cytokine release, respectively. Significantly lower numbers of CD4⁺FOXP3⁺ T cells were found in the BM of patients with RA compared to control patients with OA. High expression of CD127 (IL-7α receptor) and relatively low expression of CXCR4 (receptor for stromal cell-derived factor CXCL12) are characteristics of the CD4⁺FOXP3⁺ cells residing in the BM of RA patients. The BM-resident Tregs of RA patients demonstrated a limited suppressive activity on the investigated immune response. Our results indicate that the reduced number and impaired functional properties of CD4⁺FOXP3⁺ T cells present in the BM of RA patients may favor the inflammatory process, which is observed in RA BM.

【 授权许可】

Unknown