Immunological Separation of Bioactive Natural Compounds from Crude Drug Extract and Its Application for Cell-Based Studies

Antibodies

Shunsuke Fujii¹  Yukihiro Shoyama²  Tomoe Ohta²  Takuhiro Uto² 

[1] Faculty of Health Management, Nagasaki International University, 2825-7 Huis Ten Bosch-Cho, Sasebo 859-3298, Japan;Graduate School of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch-Cho, Sasebo 859-3298, Japan;

关键词: monoclonal antibody;    immunoaffinity column;    natural compound;    one-step separation method;    crude extract;    knockout extract;   

DOI  :  10.3390/antib10040048

来源: DOAJ

【 摘 要 】

In this study, we present a review on a useful approach, namely, immunoaffinity column coupled with monoclonal antibodies (MAbs), to separate natural compounds and its application for cell-based studies. The immunoaffinity column aids in separating the specific target compound from the crude extract. The column capacity was stable even after more than 10 purification cycles of use under the same conditions. After applying the crude extract to the column, the column was washed with washing buffer and eluted with elution buffer. The elution fraction contained the target compound bound to MAb, whereas the washing fraction was the crude extract, which contained all compounds except a group of target compounds; therefore, the washing fraction was referred to as a knockout (KO) crude extract. Cell-based studies using the KO extract revealed the actual effects of the natural compounds in the crude extract. One-step separation of natural compounds using the immunoaffinity column coupled with MAbs may help in determining the potential functions of natural compounds in crude extracts.

【 授权许可】

Unknown