Molecular Evidence of the Inhibitory Potential of Melatonin against NaAsO2-Induced Aging in Male Rats

Molecules

Roham Foroumadi¹  Hamed Haghi-Aminjan²  Seyed Mojtaba Daghighi³  Mahban Rahimifard³  Zahra Bayrami³  Madiha Khalid³  Mohammad Abdollahi³  Tina Didari³  Maryam Baeeri³  Solmaz Mohammadi-Nejad³  Ramtin Farhadi³  Mahdi Gholami³ 

[1] Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran 13145784, Iran;Pharmaceutical Sciences Research Center, Ardabil University of Medical Sciences, Ardabil 5618953141, Iran;Toxicology and Diseases Group, Pharmaceutical Sciences Research Center (PSRC), The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Tehran 1417613151, Iran;

关键词: aging;    Klotho;    Melatonin;    sodium arsenite;    TERT;    TRADD;   

DOI  :  10.3390/molecules26216603

来源: DOAJ

【 摘 要 】

Arsenic (As) poisoning is widespread due to exposure to pollution. The toxic level of (As) causes oxidative stress-induced aging and tissue damage. Since melatonin (MLT) has anti-oxidant and anti-aging properties, we aimed to evaluate the protective effect of MLT against the toxicity of sodium arsenite (NaAsO₂). Healthy male NMRI mice were divided into eight different groups. The control group received a standard regular diet. Other groups were treated with varying diets, including MLT alone, NaAsO₂, and NaAsO₂ plus MLT. After one month of treatment, biochemical and pathological tests were performed on blood, heart, and lung tissue samples. NaAsO₂ increased the levels of TNF-α, 8-hydroxy-2-deoxy guanosine (8OHdG), malondialdehyde (MDA), reactive oxygen species (ROS), and high mobility group box 1 (HMGB1), increased the expression of TNF receptor type 1-associated death domain (TRADD) mRNA and telomerase reverse transcriptase, and decreased the expression of Klotho (KL) mRNA in both plasma and tissues. In contrast, MLT reduced MDA, ROS, HMGB1, lactate, and TNF-α enhanced the mRNA expression of KL, and suppressed the mRNA expression of the TERT and TRADD genes. Thus, MLT confers potent protection against NaAsO₂- induced tissue injury and oxidative stress.

【 授权许可】

Unknown