Molecular Characterization of Chelonid Alphaherpesvirus 5 in a Black Turtle (Chelonia mydas) Fibropapilloma from Baja California Sur, Mexico

Animals

Eduardo Reséndiz¹  A.Alonso Aguirre²  AmellyHyldaí Ramos-Díaz³  Agnese Mancini⁴  Helena Fernández-Sanz⁵  AlanA. Zavala-Norzagaray⁶  JoséFrancisco Domínguez-Contreras⁷ 

[1] Departamento Académico de Ciencias Marinas y Costeras, Universidad Autónoma de Baja California Sur (UABCS), La Paz 23080, Mexico;Department of Environmental Science and Policy, George Mason University, Fairfax, VA 22030, USA;Facultad de Biología, Universidad Veracruzana, Xalapa 91000, Mexico;Grupo Tortuguero de las Californias A.C., La Paz 23098, Mexico;Health Assessments in Sea Turtles from BCS, La Paz 23085, Mexico;Instituto Politecnico Nacional, CIIDIR, Guasave 07738, Mexico;Instituto Politécnico Nacional, CICIMAR, La Paz 23096, Mexico;

关键词: Chelonia mydas;    chelonid alphaherpesvirus 5;    fibropapillomatosis;    polymerase chain reaction;   

DOI  :  10.3390/ani11010105

来源: DOAJ

【 摘 要 】

During routine monitoring in Ojo de Liebre Lagoon, Mexico, a juvenile black turtle (Chelonia mydas) was captured, physically examined, measured, weighed, sampled, and tagged. The turtle showed no clinical signs suggestive of disease. Eleven months later, this turtle was recaptured in the same area, during which one lesion suggestive of fibropapilloma on the neck was identified and sampled for histopathology and molecular analysis. Histopathology revealed hyperkeratosis, epidermal hyperplasia, acanthosis, papillary differentiation and ballooning degeneration of epidermal cells, increased fibroblasts in the dermis, and angiogenesis, among other things. Hematological values were similar to those reported for clinically healthy black turtles and did not show notable changes between the first capture and the recapture; likewise, clinicopathological evaluation did not show structural or functional damage in the turtle’s systems. The chelonid alphaherpesvirus 5 (ChHV5) UL30 gene was amplified and sequenced for phylogeny; Bayesian reconstruction showed a high alignment with the genus Scutavirus of the Eastern Pacific group. This is one of the first reports of ChHV5 in a cutaneous fibropapilloma of a black turtle in the Baja California peninsula.

【 授权许可】

Unknown