Genome-Wide Association Study Identifies 12 Loci Associated with Body Weight at Age 8 Weeks in Korean Native Chickens

Genes

Jihye Cha¹  Nayeon Kim¹  Ju-Whan Son¹  Mi-Rim Park¹  Jong-Eun Park¹  Gul Won Jang²  Krishnamoorthy Srikanth³  Seung-Hwan Lee⁴  Hyojun Choo⁵ 

[1] Animal Genome & Bioinformatics, National Institute of Animal Science, RDA, Wanju 55365, Korea;Dairy Science Division, National Institute of Animal Science, RDA, Cheonan 31000, Korea;Department of Animal Science, Cornell University, Ithaca, NY 14853, USA;Division of Animal and Dairy Science, Chungnam National University, Daejeon 34134, Korea;Poultry Research Institute, National Institute of Animal Science, RDA, Pyeongchang 25342, Korea;

关键词: Korean native chicken;    genome-wide association study;    body weight;    gene set enrichment analysis;   

DOI  :  10.3390/genes12081170

来源: DOAJ

【 摘 要 】

Meat from Korean native chickens (KNCs) has high consumer demand; however, slow growth performance and high variation in body weight (BW) of KNCs remain an issue. Genome-wide association study (GWAS) is a powerful method to identify quantitative trait-associated genomic loci. A GWAS, based on a large-scale KNC population, is needed to identify underlying genetic mechanisms related to its growth traits. To identify BW-associated genomic regions, we performed a GWAS using the chicken 60K single nucleotide polymorphism (SNP) panel for 1328 KNCs. BW was measured at 8 weeks of age, from 2018 to 2020. Twelve SNPs were associated with BW at the suggestive significance level (p < 2.95 × 10⁻⁵) and located near or within 11 candidate genes, including WDR37, KCNIP4, SLIT2, PPARGC1A, MYOCD and ADGRA3. Gene set enrichment analysis based on the GWAS results at p < 0.05 (1680 SNPs) showed that 32 Gene Ontology terms and two Kyoto Encyclopedia of Genes and Genomes pathways, including regulation of transcription, motor activity, the mitogen-activated protein kinase signaling pathway, and tight junction, were significantly enriched (p < 0.05) for BW-associated genes. These pathways are involved in cell growth and development, related to BW gain. The identified SNPs are potential biomarkers in KNC breeding.

【 授权许可】

Unknown