Luteolin decreases the pathogenicity of Aeromonas hydrophila via inhibiting the activity of aerolysin

Virulence

Shun Zhou¹  Qiuhong Yang¹  Yibin Yang¹  Yongtao Liu¹  Xiaohui Ai¹  Ning Xu¹  Lushan Zhang¹  Jing Dong¹ 

[1] Chinese Academy of Fishery Sciences;

关键词: aeromonas hydrophila;    aerolysin;    luteolin;    anti-virulence;    molecular dynamics;   

DOI  :  10.1080/21505594.2020.1867455

来源: DOAJ

【 摘 要 】

Aeromonas hydrophila (A. hydrophila) can cause a number of diseases in both human and animals. A. hydrophila-related infections in aquaculture cause severe economic losses every year throughout the world. The emergence of antibiotic resistance that is due to the abuse of antibiotics has limited the application of antibiotics. Thus, novel approaches are needed to combat with treatment failure of antibiotics caused by resistant bacterial strains. Aerolysin plays a critical role in the pathogenesis of A. hydrophila and has been considered as a novel target for developing drugs based on anti-virulence strategies. Here, we reported that luteolin, a natural product with no anti-A. hydrophila activity, could reduce aerolysin-induced hemolysis by inhibiting aerolysin activity. The binding mode was simulated by molecular docking and dynamics simulation. Then the main binding sites were confirmed by fluorescence quenching assays. We found that luteolin could hindered the formation of functional heptamer of aerolysin according to the results of the oligomerization assay. Moreover, luteolin could protect A549 cells from aerolysin mediated cell death and increase the survival rate of A. hydrophila-infected channel catfish. These findings suggest a novel approach to developing drugs fighting against A. hydrophila, and luteolin can be a promising drug candidate for treatment of A. hydrophila-associated infections.

【 授权许可】

Unknown