Immunomorphological Pattern of Molecular Chaperones in Normal and Pathological Thyroid Tissues and Circulating Exosomes: Potential Use in Clinics

International Journal of Molecular Sciences

Celeste Caruso Bavisotto¹  Fabio Bucchieri¹  Francesca Rappa¹  Claudia Campanella¹  Antonella Marino Gammazza¹  Giovanni Tomasello¹  Alessandro Pitruzzella¹  Rosario Barone¹  Francesco Cappello¹  Calogero Cipolla²  Giuseppa Graceffa²  Rossana Porcasi³  Daniela Cabibi³  AlbertoJ. L. Macario⁴  Everly Conway de Macario⁴  PierLuigi San Biagio⁵  Donatella Bulone⁵ 

[1] Department of Biomedicine, Neuroscience and Advanced Diagnostics (BIND), Institute of Human Anatomy and Histology, University of Palermo, 90127 Palermo, Italy;Department of Surgical Oncology and Oral Sciences, University of Palermo, 90127 Palermo, Italy;Department “G. D’Alessandro”, Pathology Institute, University of Palermo, 90127 Palermo, Italy;Euro-Mediterranean Institute of Science and Technology (IEMEST), 90100 Palermo, Italy;Institute of Biophysics, National Research Council, 90100 Palermo, Italy;

关键词: thyroid gland;    papillary carcinoma;    molecular chaperones;    heat shock proteins (Hsp);    goiter;    exosomes;    diagnosis;   

DOI  :  10.3390/ijms20184496

来源: DOAJ

【 摘 要 】

The thyroid is a major component of the endocrine system and its pathology can cause serious diseases, e.g., papillary carcinoma (PC). However, the carcinogenic mechanisms are poorly understood and clinical useful biomarkers are scarce. Therefore, we determined if there are quantitative patterns of molecular chaperones in the tumor tissue and circulating exosomes that may be useful in diagnosis and provide clues on their participation in carcinogenesis. Hsp27, Hsp60, Hsp70, and Hsp90 were quantified by immunohistochemistry in PC, benign goiter (BG), and normal peritumoral tissue (PT). The same chaperones were assessed in plasma exosomes from PC and BG patients before and after ablative surgery, using Western blotting. Hsp27, Hsp60, and Hsp90 were increased in PC in comparison with PT and BG but no differences were found for Hsp70. Similarly, exosomal levels of Hsp27, Hsp60, and Hsp90 were higher in PC than in BG, and those in PC were higher before ablative surgery than after it. Hsp27, Hsp60, and Hsp90 show distinctive quantitative patterns in thyroid tissue and circulating exosomes in PC as compared with BG, suggesting some implication in the carcinogenesis of these chaperones and indicating their potential as biomarkers for clinical applications.

【 授权许可】

Unknown