Prevalence of some virulence factors and genotyping of hospital-acquired uropathogenic Escherichia coli isolates recovered from cancer patients

Journal of Global Antimicrobial Resistance

Adel Gabr¹  Mohamed Taha Salim²  Ahmed Talaat Mahmoud²  Hamada Mohamed Halby²  Reham Ali Ibrahem³ 

[1] Department of Medical Oncology and Malignant Hematology, South Egypt Cancer Institute, Assuit University, Assuit 71515, Egypt;Department of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar University, Assuit 71524, Egypt;Department of Microbiology and Immunology, Faculty of Pharmacy, El-Minia University, Minia 61111, Egypt;

关键词: Cancer patients;    Uropathogenic Escherichia coli;    UPEC;    Virulence factor;    ERIC-PCR;   

DOI  :  

来源: DOAJ

【 摘 要 】

Objectives: This study aimed to determine the prevalence of virulence factors among uropathogenic Escherichia coli (UPEC) isolates from cancer patients and to investigate their genetic diversity using ERIC-PCR. Methods: A total of 42 E. coli were recovered from urine samples from cancer patients admitted to Assiut University Hospital. PCR was used to detect the presence of three virulence genes (papC, iutA and cnf1). Genetic diversity of the isolates was determined using the ERIC-PCR fingerprinting method, and amplified products were separated by agarose gel electrophoresis. Gel electrophoresis banding patterns were used for dendrogram generation using NTSYSpc software. Results: Among the 42 UPEC isolates, papC was the most common virulence gene (55% of isolates), followed by iutA (38%) and cnf1 (2%). ERIC-PCR successfully produced multiple amplicons (range 2–11 bands) in each strain, with molecular weights ranging from 285 to 3000 bp. Some UPEC isolates had identical ERIC-PCR profiles (identical banding patterns), whilst 22 UPEC isolates had different ERIC-PCR profiles. The phylogenetic dendrogram of ERIC-PCR showed that the 42 isolates can be differentiated into three major clusters (I, II and III), with cluster I representing 76% of isolates, cluster II representing 19% and cluster III representing 5%. Conclusions: The results of this study suggest that both papC and iutA genes may have an important role in the pathogenesis of overt urinary tract infection. Dendrogram analysis of the ERIC-PCR profiles revealed that all UPEC isolates were assigned into three main clusters, indicating the spread of distinct clonal groups that are responsible for hospital-acquired infections.

【 授权许可】

Unknown