ACTIVITY DEPENDENCE OF CELLULOLYTIC ENZYMES MONOAND DIKARYOTIC CULTURES OF Stereum hirsutum (Wild.) Gray (Basidiomycetes) FROM A SUBSTRATUM

Biotechnologia Acta

S. M. Boiko¹  M. E. Ryazanova¹ 

[1] Donetsk national university, Ukraine;

关键词: cellulolytic enzymes;    Stereum hirsutum;    monokaryotic cultures;    electrophoresis of proteins.;   

DOI  :  10.15407/biotech6.03.116

来源: DOAJ

【 摘 要 】

Activity of cellulolytic enzymes cultures Stereum hirsutum (Wild.) Gray, different nuclear status, depending on the substrate have been studied. The most productive by synthesis endoglucanases monokaryotic cultures of Sh1-4 and Sh7-2 were determined. It is found that nutrient medium containing filtering paper is optimal for synthesis of cellulase (culture Sh1-4, maximum of cellulolytic activity is 319 ?М/mg, culture Sh7-2 — 101 ?М/mg). Maximum of cellulolytic activity at cultivation with sawdust of trees for culture Sh1-4 observed at the 10th day was 142 ?М/mg (oak sawdust), for culture Sh7-2 at the 20th days it was 34 ?М/mg (ash-tree sawdust). Study of quality cellulolytic enzymes enabled to establishe presence of four exoenzymes of Cx complex with a relative electrophoretic mobility of 0.22; 0.24; 0.47; 0.74. Enzyme with relative electrophoretic mobility 0.47 showed high concentration and activity.

【 授权许可】

Unknown