Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffee

Pesquisa Agropecuária Brasileira

Lilian Padilha¹  Carlos Henrique Siqueira de Carvalho¹  Mirian Peres Maluf¹  Gabriella Santos Pereira²  Edila Vilela Resende Von Pinho²  Rita de Kássia Siqueira Teixeira²  Bruna Line de Carvalho² 

[1] ;Universidade Federal de Lavras;

关键词: Coffea arabica;    Leucoptera coffeella;    EST-SSR;    genótipos;   

DOI  :  10.1590/S0100-204X2011001200010

来源: DOAJ

【 摘 要 】

The objective of this work was to analyze coffee (Coffea arabica) genotypes resistant to the coffee leaf miner (Leucoptera coffeella) using microsatellite markers. Sixty-six loci were evaluated, of which 63 were obtained from the Brazilian Coffee Expressed Sequence Tag (EST) database. These loci were amplified in bulks of individuals from F5 progenies of 'Siriema' (C. arabica x C. racemosa) resistant and susceptible to the insect, in eight samples of C. racemosa, and in a F6 population of 'Siriema' with 91 individuals segregating for resistance to the leaf miner. Polymorphisms were verified for two simple sequence repeat (SSR) loci in bulks of the susceptible progenies. The two polymorphic alleles were present in around 70% of the susceptible genotypes in F5 and in approximately 90% of the susceptible individuals in F6. However, the polymorphic EST-SSR markers among populations contrasting for resistance to leaf miner were not correlated to the evaluated characteristics. SSR markers show inter- and intraspecific polymorphism in C. arabica and C. racemosa.

【 授权许可】

Unknown