Identification of the Bovine Growth Hormone Gene and Alui Loci Polymorphism by PCR – RFLP Method

Scientific Papers Animal Science and Biotechnologies

Anna Trakovická¹  Nina Moravčíková¹ 

[1] Slovak University of Agriculture in Nitra, Nitra;

关键词: cattle;    growth hormone gene;    pcr-rflp;    polymorphism;   

DOI  :  

来源: DOAJ

【 摘 要 】

AbstractThe aim of this study was detection of polymorphism in the bovine growth hormone gene using PCR – RFLP asan efficient method for genetic analysis of polymorphism. A polymorphic site of the growth hormone gene (AluIpolymorphism) that results in an amino acid change at position 127 of the protein chain (leucine, L to valine, V) hasbeen linked to differences in circulating metabolites and metabolic hormones and to milk yield traits. The growthhormone gene is a candidate gene for body weight and weight gain in cattle since it plays a fundamental role ingrowth regulation. The polymorphism of growth hormone gene was studied in a group of 58 bulls of Slovak spottedbreed. A strategy employing PCR was used to amplify 428 bp products from blood samples. Digestion of PCRproducts with restriction enzyme AluI revealed two alleles: allele L was 265, 96, 51, 16 fragments and allele V was265, 147 and 16. Three patterns were observed and with frequencies 0.404, 0.473 and 0.123 for LL, LV and VV,respectively. The frequency of alleles L was 0.6404 and V was 0.3596.

【 授权许可】

Unknown