Ultrasound-Assisted Extraction of Stilbenes from Grape Canes

Molecules

Miguel Palma¹  Zulema Piñeiro²  Maria Jose Serrano²  Almudena Marrufo-Curtido² 

[1] Departamento de Química Analítica, IVAGRO, Universidad de Cadiz, Apdo. 40, Puerto Real 11510, Spain;IFAPA Rancho de la Merced, Carretera de Trebujena, Km. 2.2, Apdo. 589, Jerez de la Frontera 11471, Spain;

关键词: trans-resveratrol;    stilbenes;    ultrasound;    extraction;    HPLC;    grape canes;   

DOI  :  10.3390/molecules21060784

来源: DOAJ

【 摘 要 】

An analytical ultrasound-assisted extraction (UAE) method has been optimized and validated for the rapid extraction of stilbenes from grape canes. The influence of sample pre-treatment (oven or freeze-drying) and several extraction variables (solvent, sample-solvent ratio and extraction time between others) on the extraction process were analyzed. The new method allowed the main stilbenes in grape canes to be extracted in just 10 min, with an extraction temperature of 75 °C and 60% ethanol in water as the extraction solvent. Validation of the extraction method was based on analytical properties. The resulting RSDs (n = 5) for interday/intraday precision were less than 10%. Furthermore, the method was successfully applied in the analysis of 20 different grape cane samples. The result showed that grape cane byproducts are potentially sources of bioactive compounds of interest for pharmaceutical and food industries.

【 授权许可】

Unknown