Super-Resolution Imaging Approaches for Quantifying F-Actin in Immune Cells

Frontiers in Cell and Developmental Biology

Evelyn Garlick¹  Steven G. Thomas¹  Dylan M. Owen² 

[1] Centre of Membrane Proteins and Receptors, University of Birmingham and University of Nottingham, Midlands, United Kingdom;Institute for Immunology and Immunotherapy, College of Medical and Dental Science and School of Mathematics, College of Engineering and Physical Science, University of Birmingham, Birmingham, United Kingdom;Institute of Cardiovascular Sciences, College of Medical and Dental Science, University of Birmingham, Birmingham, United Kingdom;

关键词: actin;    microscopy;    super-resolution;    fiber analysis;    cytoskeleton;   

DOI  :  10.3389/fcell.2021.676066

来源: DOAJ

【 摘 要 】

Immune cells comprise a diverse set of cells that undergo a complex array of biological processes that must be tightly regulated. A key component of cellular machinery that achieves this is the cytoskeleton. Therefore, imaging and quantitatively describing the architecture and dynamics of the cytoskeleton is an important research goal. Optical microscopy is well suited to this task. Here, we review the latest in the state-of-the-art methodology for labeling the cytoskeleton, fluorescence microscopy hardware suitable for such imaging and quantitative statistical analysis software applicable to describing cytoskeletal structures. We also highlight ongoing challenges and areas for future development.

【 授权许可】

Unknown