Evaluation of the Potential of Collagen from Codfish Skin as a Biomaterial for Biomedical Applications

Marine Drugs

AnaM. Carvalho¹  AlexandraP. Marques¹  RuiL. Reis¹  TiagoH. Silva¹ 

[1] 3B’s Research Group, I3Bs–Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence in Tissue Engineering and Regenerative Medicine Avepark–Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, Portugal;

关键词: marine-origin collagen;    codfish;    biophysical characterization;    biologic activity;    ASTM guidelines;    biomedical application;    marine biomaterials;   

DOI  :  10.3390/md16120495

来源: DOAJ

【 摘 要 】

Collagen is one of the most widely used biomaterials, not only due its biocompatibility, biodegradability and weak antigenic potential, but also due to its role in the structure and function of tissues. Searching for alternative collagen sources, the aim of this study was to extract collagen from the skin of codfish, previously obtained as a by-product of fish industrial plants, and characterize it regarding its use as a biomaterial for biomedical application, according to American Society for Testing and Materials (ASTM) Guidelines. Collagen type I with a high degree of purity was obtained through acid-extraction, as confirmed by colorimetric assays, SDS-PAGE and amino acid composition. Thermal analysis revealed a denaturing temperature around 16 °C. Moreover, collagen showed a concentration-dependent effect in metabolism and on cell adhesion of lung fibroblast MRC-5 cells. In conclusion, this study shows that collagen can be obtained from marine-origin sources, while preserving its bioactivity, supporting its use in biomedical applications.

【 授权许可】

Unknown