BIOCHEMICAL CHANGES IN CATTLE BODY AT FASCIOLOSIS

Российский паразитологический журнал

I. D. Shelyakin¹  S. N. Semyonov¹  O. M. Marmurova¹ 

[1] Voronezh State Agricultural University named after Emperor Peter the Great 394087, Voronezh, 1 Michurin st.;

关键词: cattle;    fasciolosis;    enzymes;    total protein;    carbohydrate metabolism;    lipid metabolism;   

DOI  :  

来源: DOAJ

【 摘 要 】

Functioning of a number of transamination enzymes and alkaline phosphatase as well as some values of carbohydrate and lipid metabolism on background of protein in cattle blood at fasciolosis are studied. Total blood protein was determined by Loury method, alkaline phosphatase activity – by colorimetric method based on the hydrolysis of n-nitrophenol phosphate. The glutamate transferase activity was estimated by L.J. Olsen method, transaminase activity – by Reitman-Frankel method. Cholesterol levels were measured according to Liebermann–Burchard reaction, the pyruvate content was determined using the diphenylhydrazine method, and lactate – according to reaction withparaoxydiphenyl. The functional status of blood at fasciolosis is marked by the decrease in total protein and pyruvate with the increase of activity of glutamate transferase, alkaline phosphatase, lactate and cholesterol.

【 授权许可】

Unknown