Mechanotransduction in hippocampal neurons operates under localized low picoNewton forces

iScience

Simone Mortal¹  Ulisse Bocchero²  Zhongjie Ye²  Yunzhen Li³  Vincent Torre⁴  Dan Cojoc⁴  Fabio Falleroni⁵ 

[1] Corresponding author;Neuroscience Area, International School for Advanced Studies (SISSA), 34136 Trieste, Italy;National Eye Institute (NEI), Bethesda, MD 20892, USA;Neuroscience Area, International School for Advanced Studies (SISSA), 34136 Trieste, Italy;Technische Universitat Munchen Fakultat fur Physik (TUM), 85748 Garching, Germany;

关键词: Mechanobiology;    Biological sciences;    Neuroscience;    Cellular neuroscience;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: There is growing evidence suggesting that mechanical properties of CNS neurons may play an important regulatory role in cellular processes. Here, we employ an oscillatory optical tweezers (OOT) to exert a local indentation with forces in the range of 5–50 pN. We found that single local indentation above a threshold of 13 ± 1 pN evokes a transient intracellular calcium change, whereas repeated mechanical stimulations induce a more sustained and variable calcium response. Importantly, neurons were able to differentiate the magnitude of mechanical stimuli. Chemical perturbation and whole-cell patch clamp recordings suggest that mechanically evoked response requires the influx of extracellular calcium through transmembrane ion channels. Moreover, we observed a mechanically evoked activation of the CAMKII and small G protein RhoA. These results all together suggest that mechanical signaling among developed neurons fully operates in neuronal networks under physiological conditions.

【 授权许可】

Unknown