Functional Effects of Receptor-Binding Domain Mutations of SARS-CoV-2 B.1.351 and P.1 Variants

Frontiers in Immunology

Henning Bundgaard¹  Ruth Frikke-Schmidt²  Peter Garred³  Susanne Dam Nielsen³  Kasper Karmark Iversen⁴  Mikkel-Ole Skjoedt⁶  Cecilie Bo Hansen⁷  Ida Jarlhelt⁷  Rafael Bayarri-Olmos⁷  Charlotte Helgstrand⁸  Jais Rose Bjelke⁸  Finn Matthiesen⁸  Laust Bruun Johnsen⁸  Sisse Rye Ostrowski⁹ 

[1] Department of Cardiology, Rigshospitalet, Copenhagen, Denmark;Department of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark;Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark;Department of Emergency Medicine, Herlev and Gentofte Hospital, Copenhagen, Denmark;Department of Infectious Diseases, Rigshospitalet, Copenhagen, Denmark;Institute of Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark;Laboratory of Molecular Medicine, Department of Clinical Immunology, Section 7631, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark;Recombinant Technologies, Novo Nordisk A/S, Måløv, Denmark;The Blood Bank, Department of Clinical Immunology, Section 2034, Rigshospitalet, Copenhagen, Denmark;

关键词: SARS-CoV-2;    RBD;    ACE-2;    B.1.351;    P.1;    variant of concern;   

DOI  :  10.3389/fimmu.2021.757197

来源: DOAJ

【 摘 要 】

The recent identification and rise to dominance of the P.1 and B.1.351 SARS-CoV-2 variants have brought international concern because they may confer fitness advantages. The same three positions in the receptor-binding domain (RBD) are affected in both variants, but where the 417 substitution differs, the E484K/N501Y have co-evolved by convergent evolution. Here we characterize the functional and immune evasive consequences of the P.1 and B.1.351 RBD mutations. E484K and N501Y result in gain-of-function with two different outcomes: The N501Y confers a ten-fold affinity increase towards ACE-2, but a modest antibody evasion potential of plasma from convalescent or vaccinated individuals, whereas the E484K displays a significant antibody evasion capacity without a major impact on affinity. On the other hand, the two different 417 substitutions severely impair the RBD/ACE-2 affinity, but in the combined P.1 and B.1.351 RBD variants, this effect is partly counterbalanced by the effect of the E484K and N501Y. Our results suggest that the combination of these three mutations is a two-step forward and one step back in terms of viral fitness.

【 授权许可】

Unknown