Rate of Freeze Impacts the Survival and Immune Responses Post Cryoablation of Melanoma

Frontiers in Immunology

Lana E. Kandalaft¹  Cheryl Lai-Lai Chiang²  Rafael Duran³  Chakradhar Yakkala³  Alban Denys³  Christine Sempoux⁴  Julien Dagher⁴  Bhanu Koppolu⁵ 

[1] Center of Experimental Therapeutics, Department of Oncology, Lausanne University Hospital, Lausanne, Switzerland;Department of Oncology, Lausanne University Hospital, Ludwig Institute for Cancer Research, University of Lausanne, Lausanne, Switzerland;Department of Radiology and Interventional Radiology, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland;Institute of Pathology, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland;Interventional Oncology and Immuno-oncology, BTG/Boston Scientific, Natick, MA, United States;

关键词: cryoablation;    melanoma;    immunotherapy;    immune response;    tumors;   

DOI  :  10.3389/fimmu.2021.695150

来源: DOAJ

【 摘 要 】

The emergence of ablative therapies has revolutionized the treatment of inoperable solid tumors. Cryoablation stands out for its uniqueness of operation based on hypothermia, and for its ability to unleash the native tumor antigens, resulting in the generation of anti-tumor immune responses. It is not clearly understood how alterations in the rate of freeze impact the immune response outcomes. In this study, we tested fast freeze and slow freeze rates for their locoregional effectiveness and their ability to elicit immune responses in a B16F10 mouse model of melanoma. Tumor bearing mice treated with fast freeze protocol survived better than the ones treated with slow freeze protocol. Fast freeze resulted in a higher magnitude of CD4+ and CD8+ T-cell responses, and a significantly extended survival post re-challenge. Thus, fast freeze rate should be applied in any future studies employing cryoablation as an in vivo vaccination tool in conjunction with targeted immunotherapies.

【 授权许可】

Unknown