Cofilin Phosphorylation Mediates Proliferation in Response to Platelet-Derived Growth Factor-BB in Rat Aortic Smooth Muscle Cells

Journal of Pharmacological Sciences

Hyung-Kwan Jang¹  Pyo-Jam Park²  Taekyu Park²  Soon Heum Kim³  Hwan Myung Lee³  Bokyung Kim³  Sun-Jong Kim³  Wahn Soo Choi³  Seung Hwa Park³  Chang-Kwon Lee³  Kyung-Jong Won³ 

[1] Department of Microbiology, College of Veterinary Medicine, Chonbuk National University, 664-14 Deokjin-dong, Jeonju 561-756, Korea;Division of Life Science, College of Biomedical and Health Science, Konkuk University, 322 Danwol-dong, Chungju 380-701, Korea;Institute of Medical Sciences, School of Medicine, and 322 Danwol-dong, Chungju 380-701, Korea;

DOI  :  

来源: DOAJ

【 摘 要 】

Cofilin, an actin-binding protein, is essential for a variety of cell responses. In this study, we investigated the correlation between proliferation and cofilin phosphorylation in response to platelet-derived growth factor (PDGF) in rat aortic smooth muscle cells (RASMCs). The phosphorylation of cofilin and activity of mitogen-activated protein kinase (MAPK) were measured by Western analyses and proliferation in RASMCs was measured by BrdU incorporation assays. The phosphorylation of cofilin in RASMCs was decreased by PDGF-BB treatment at 10 min, but recovered to the level of the quiescent state at 60 min. PDGF-BB–induced dephosphorylation of cofilin was inhibited by pretreatment with piceatannol (a spleen tyrosine kinase [Syk] inhibitor), PP2 (a Src inhibitor), or SP600125 (a c-Jun N-terminal kinase [JNK] inhibitor), but not by PD98059, an inhibitor of extracellular signal-regulated kinase 1 /2. PDGF-BB increased JNK activity and proliferation, and these responses were suppressed by kinase inhibitors and small interference RNA-cofilin. The results suggest that PDGF-BB–induced dephosphorylation of cofilin can be promoted via the JNK pathway, which is regulated by both Syk and Src kinases and that cofilin dephosphorylation may be involved in PDGF-BB–induced RASMC proliferation. Keywords:: cofilin, proliferation, rat aortic smooth muscle cell (RASMC), platelet-derived growth factor-BB (PDGF-BB), c-Jun N-terminal kinase (JNK)

【 授权许可】

Unknown