Study on protective effect of ketogenic diet against hippocampal neurons of kainic acid-induced epileptic rats

Chinese Journal of Contemporary Neurology and Neurosurgery

Xi-ke ZHU¹  Nan-nan ZHANG²  Xue-mei FENG²  Xiu-ying FANG²  Lin CONG³ 

[1] Core-Facility for Medical Research, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning, China;Department of Neuroelectrophysiology, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning, China;Department of Neurology, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning, China;

关键词: Epilepsy;    Kainic acid;    Diet therapy;    Ketone bodies;    Hippocampus;    Neurons;    Disease models, animal;   

DOI  :  

来源: DOAJ

【 摘 要 】

Objective To investigate the protective effects ofketogenic diet on hippocampal neurons of kainic acid (KA)-induced epilepticrats.  Methods A total of 40 healthy male specific pathogenfree (SPF) Sprague-Dawley (SD) rats were randomly divided into 4 groups, witheach group containing 10 rats. Epileptic rat models were formed by injection ofKA through lateral ventricle with brain stereotactic instrument. According toRacine classification, epileptic seizures in rats above grade Ⅳ were definedsuccessful. Then the rats were given different dietary treatment: Group C withnormal saline and normal diet, Group K with normal saline and ketogenic diet,Group E with KA and normal diet, Group EK with KA and ketogenic diet. All ratswere observed for 21 d, and were recorded each body weight. The epilepticseizure frequency and duration were observed at 12:00-15:00 daily. At the 21stday, all rats were put to death, and the brain hippocampus tissues wereseparated. Neuron injury of rat hippocampal CA3 region in Group E and EK wasobserved by HE staining. Normal neuron number of rat hippocampal CA3 region inGroup E and EK was counted by Nissl staining. Results Group Cand K had no epileptic seizures, and the neuron number in hippocampal CA3 regionwas normal. Rats in both Group E and EK had grade Ⅳ or Ⅴ seizures. The number ofseizures in Group EK [(17.90 ± 4.12) times] after 21-day ketogenic diet wasdecreased significantly compared to Group E [(30.50 ± 4.40) times] after 21-daynormal diet (t = 6.606, P = 0.000). The seizure duration inGroup EK [(212.70 ± 17.75) s] after 21-day ketogenic diet was shortened comparedto Group E [(335.00 ± 14.21) s] after 21-day normal diet (t = 17.011,P = 0.000). The normal neuron number in hippocampal CA3 region in GroupEK (117.67 ± 7.51) was more than those in Group E (71.33 ± 6.11), withstatistically significant difference (t = 9.614, P = 0.000). Conclusions Ketogenic diet has protective effect onhippocampal neurons of kainic acid-induced epileptic rats.

 

DOI: 10.3969/j.issn.1672-6731.2015.11.011

【 授权许可】

Unknown