In Vitro and In Vivo Biocompatibility Studies of a Cast and Coated Titanium Alloy

Molecules

Sabine Wenisch¹  Reinhard Schnettler²  Volker Alt³  Ulrich Thormann⁴  Lucie de Azevedo⁴  Christian Heiss⁴  Ursula Sommer⁴  Stephan Laurich⁴  Katharina Viehoff⁴ 

[1] Clinic of Small Animals, c/o Institute of Veterinary Anatomy, Histology and Embryology, Justus-Liebig-University Giessen, Frankfurter Strasse 98, 35392 Giessen, Germany;Department of Oral and Maxillofacial Surgery, Division for Regenerative Orofacial Medicine, University Hospital Hamburg-Eppendorf, 20246 Hamburg, Germany;Department of Trauma Surgery, University Medical Center Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany;Experimental Trauma Surgery, Justus-Liebig-University Giessen, Aulweg 128 (ForMED), 35392 Giessen, Germany;

关键词: bone;    implant;    Ti-6Al-7Nb;    rat;    sheep;    cell culture;   

DOI  :  10.3390/molecules25153399

来源: DOAJ

【 摘 要 】

The biocompatibility of a cast porous and with a calcium titanate reaction layer functionalized titanium alloy (Ti-6Al-7Nb) was tested by means of cell culture, and a small (rat) and large animal (sheep) model. The uncoated titanium material served as a control. In-vitro tests included the validation of osteoblast-like cells attached to the surface of the material with scanning electron microscopy and immunofluorescence of cytoskeletal actin as well as their osteogenic development, the ability to mineralize, and their vitality. Following the in-vitro tests a small animal (rat) and big animal (sheep) model were accomplished by inserting a cylindrical titanium implant into a drill hole defect in the femoral condyle. After 7, 14, and 30 days (rat) and 6 months (sheep) the condyles were studied regarding histological and histomorphometrical characteristics. Uncoated and coated material showed a good biocompatibility both in cell culture and animal models. While the defect area in the rat is well consolidated after 30 days, the sheep show only little bone inside the implant after 6 months, possibly due to stress shielding. None of the executed methods indicated a statistically significant difference between coated and uncoated material.

【 授权许可】

Unknown