Bioconversion of Sweet Sorghum Residues by Trichoderma citrinoviride C1 Enzymes Cocktail for Effective Bioethanol Production

Catalysts

Anna Kancelista¹  Paweł Korzeniowski¹  Wojciech Łaba¹  Joanna Chmielewska² 

[1] Department of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life Sciences, 37 Chełmońskiego Street, 51-630 Wrocław, Poland;Department of Fermentation and Cereals Technology, Wroclaw University of Environmental and Life Sciences, 37 Chełmońskiego Street, 51-630 Wrocław, Poland;

关键词: lignocellulose;    waste biomass;    saccharification;    microbial fermentation;    2G ethanol;    Box–Behnken design;   

DOI  :  10.3390/catal10111292

来源: DOAJ

【 摘 要 】

Improved cost-effective bioethanol production using inexpensive enzymes preparation was investigated. Three types of waste lignocellulosic materials were converted—for the production of enzyme preparation, a mixture of sugar beet pulp and wheat bran, while the source of sugars in hydrolysates was sweet sorghum biomass. A novel enzyme cocktail of Trichoderma citrinoviride C1 is presented. The one-step ultrafiltration process of crude enzyme extract resulted in a threefold increase of cellulolytic and xylanolytic activities. The effectiveness of enzyme preparation, compared to Cellic^® CTec2, was tested in an optimized enzymatic hydrolysis process. Depending on the test conditions, hydrolysates with different glucose concentrations were obtained—from 6.3 g L⁻¹ to 14.6 g L⁻¹ (representing from 90% to 79% of the CTec2 enzyme yield, respectively). Furthermore, ethanol production by Saccharomyces cerevisiae SIHA Active Yeast 6 strain DF 639 in optimal conditions reached about 120 mL kg d.m.⁻¹ (75% compared with the CTec2 process). The achieved yields suggested that the produced enzyme cocktail C1 could be potentially used to reduce the cost of bioethanol production from sweet sorghum biomass.

【 授权许可】

Unknown