The Circ_0001367/miR-545-3p/LUZP1 Axis Regulates Cell Proliferation, Migration and Invasion in Glioma Cells

Frontiers in Oncology

Yuan Wang¹  Chaonan Zheng¹  Li Zhang¹  Peng Zhang²  Shan Cheng³  Suwen Li³  Haoran Li³  Jun Dong³  Liang Liu³  Xuchen Dong⁴ 

[1] College of Pharmaceutical Sciences, Soochow University, Suzhou, China;Department of Neurosurgery, Rugao Hospital Affiliated to Nantong University, Nantong, China;Department of Neurosurgery, Second Affiliated Hospital of Soochow University, Suzhou, China;Medical College of Soochow University, Suzhou, China;

关键词: circRNA;    hsa_circ_0001367;    miR-545-3p;    LUZP1;    glioma;   

DOI  :  10.3389/fonc.2021.781471

来源: DOAJ

【 摘 要 】

Glioma is the most common primary intracranial malignant tumour in adults. It has a high incidence and poses a serious threat to human health. Circular RNA is a hotspot of cancer research. In this study, we aimed to explore the role of circ_0001367 in gliomagenesis and the underlying mechanism. First, qRT-PCR was conducted, which showed that circ_0001367 level was downregulated in glioma tissues and cells. Next, gain-of-function and loss-of-function assays were performed, which indicated that circ_0001367 inhibited the proliferation, migration and invasion of glioma cells. Subsequent bioinformatics analysis, dual-luciferase reporter assays, RNA immunoprecipitation assays and cell function assays demonstrated that circ_0001367 inhibited the proliferation, migration and invasion of glioma cells by absorbing miR-545-3p and thereby regulating the expression of leucine zipper protein (LUZP1). Finally, an in vivo experiment was conducted, which demonstrated that circ_0001367 inhibited glioma growth in vivo by modulating miR-545-3p and LUZP1. Taken together, the results of this study demonstrate that the circ_0001367/miR-545-3p/LUZP1 axis may be a novel target for glioma therapy.

【 授权许可】

Unknown