Transcriptome Profiling and Identification of Transcription Factors in Ramie (Boehmeria nivea L. Gaud) in Response to PEG Treatment, Using Illumina Paired-End Sequencing Technology

International Journal of Molecular Sciences

Lunjin Dai¹  Xia An¹  Bo Wang¹  Jie Chen¹  Lijun Liu¹  Dingxiang Peng¹  Jingyu Zhang¹  Yiwen Liao¹ 

[1] Key Laboratory of Crop Ecophysiology and Farming Systems in the Middle Reaches ofthe Yangtze River, Ministry of Agriculture, College of Plant Science and Technology,Huazhong Agricultural University, Wuhan 430070, China;

关键词: ramie;    PEG;    paired-end sequencing;    transcription factor;   

DOI  :  10.3390/ijms16023493

来源: DOAJ

【 摘 要 】

Ramie (Boehmeria nivea L. Gaud), commonly known as China grass, is a perennial bast fiber plant of the Urticaceae. In China, ramie farming, industry, and trade provide income for about five million people. Drought stress severely affects ramie stem growth and causes a dramatic decrease in ramie fiber production. There is a need to enhance ramie’s tolerance to drought stress. However, the drought stress regulatory mechanism in ramie remains unknown. Water stress imposed by polyethylene glycol (PEG) is a common and convenient method to evaluate plant drought tolerance. In this study, transcriptome analysis of cDNA collections from ramie subjected to PEG treatment was conducted using Illumina paired-end sequencing, which generated 170 million raw sequence reads. Between leaves and roots subjected to 24 (L2 and R2) and 72 (L3 and R3) h of PEG treatment, 16,798 genes were differentially expressed (9281 in leaves and 8627 in roots). Among these, 25 transcription factors (TFs) from the AP2 (3), MYB (6), NAC (9),zinc finger (5), and bZIP (2) families were considered to be associated with drought stress. The identified TFs could be used to further investigate drought adaptation in ramie.

【 授权许可】

Unknown