Miscanthus x giganteus Stem Versus Leaf-Derived Lignins Differing in Monolignol Ratio and Linkage

International Journal of Molecular Sciences

Christopher Konow¹  XuanTung Do²  Michel Bergs²  Peter Kusch²  Margit Schulze²  Thorsten Kraska³  Georg Völkering⁴  Ralf Pude⁴  Yulia Monakhova⁵ 

[1] Department of Chemistry, MS 015, Brandeis University, 415 South Street, Waltham, MA 02453, USA;Department of Natural Sciences, Bonn-Rhein-Sieg University of Applied Sciences, von-Liebig-Strasse 20, D-53359 Rheinbach, Germany;Field Lab Campus Klein-Altendorf, Faculty of Agriculture, University of Bonn, Campus Klein-Altendorf 1, D-53359 Rheinbach, Germany;Institute of Crop Science and Resource Conservation, Faculty of Agriculture, University of Bonn, Klein-Altendorf 2, D-53359 Rheinbach, Germany;Spectral Service AG, Emil-Hoffmann-Strasse 33, D-50996 Köln, Germany;

关键词: biomass;    chemometrics;    genotype;    HSQC NMR;    lignin;    Miscanthus x giganteus;    monolignol ratio;    principal component analysis;   

DOI  :  10.3390/ijms20051200

来源: DOAJ

【 摘 要 】

As a renewable, Miscanthus offers numerous advantages such as high photosynthesis activity (as a C4 plant) and an exceptional CO2 fixation rate. These properties make Miscanthus very attractive for industrial exploitation, such as lignin generation. In this paper, we present a systematic study analyzing the correlation of the lignin structure with the Miscanthus genotype and plant portion (stem versus leaf). Specifically, the ratio of the three monolignols and corresponding building blocks as well as the linkages formed between the units have been studied. The lignin amount has been determined for M. x giganteus (Gig17, Gig34, Gig35), M. nagara (NagG10), M. sinensis (Sin2), and M. robustus (Rob4) harvested at different time points (September, December, and April). The influence of the Miscanthus genotype and plant component (leaf vs. stem) has been studied to develop corresponding structure-property relationships (i.e., correlations in molecular weight, polydispersity, and decomposition temperature). Lignin isolation was performed using non-catalyzed organosolv pulping and the structure analysis includes compositional analysis, Fourier transform infradred (FTIR), ultraviolet/visible (UV-Vis), hetero-nuclear single quantum correlation nuclear magnetic resonsnce (HSQC-NMR), thermogravimetric analysis (TGA), and pyrolysis gaschromatography/mass spectrometry (GC/MS). Structural differences were found for stem and leaf-derived lignins. Compared to beech wood lignins, Miscanthus lignins possess lower molecular weight and narrow polydispersities (<1.5 Miscanthus vs. >2.5 beech) corresponding to improved homogeneity. In addition to conventional univariate analysis of FTIR spectra, multivariate chemometrics revealed distinct differences for aromatic in-plane deformations of stem versus leaf-derived lignins. These results emphasize the potential of Miscanthus as a low-input resource and a Miscanthus-derived lignin as promising agricultural feedstock.

【 授权许可】

Unknown