PEG 6000-Stimulated Drought Stress Improves the Attributes of In Vitro Growth, Steviol Glycosides Production, and Antioxidant Activities in Stevia rebaudiana Bertoni

Plants

Muhammad Adeel¹  Rabia Javed²  Muhammad Rizwan³  MuhammadArslan Ahmad⁴  Yuesuo Yang⁴ 

[1] Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation, College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China;Department of Tissue Engineering, China Medical University, Shenyang 110122, China;Institute of Soil Science, PMAS Arid Agriculture University Rawalpindi, Rawalpindi 46000, Pakistan;Key Lab of Eco-Restoration of Regional Contaminated Environment, Ministry of Education, Shenyang University, Shenyang 110044, China;

关键词: Stevia rebaudiana;    steviol glycosides (SGs);    antioxidant activities;    polyethylene glycol (PEG);    drought stress;   

DOI  :  10.3390/plants9111552

来源: DOAJ

【 摘 要 】

This study addresses the favourable effects of drought stress imposed by polyethylene glycol (PEG) 6000 on the micropropagated shoots of Stevia rebaudiana. Various concentrations, i.e., 0, 0.5, 1, 2, and 4% of PEG 6000 were applied to the nodal shoot explants for four weeks, and the influence produced on shoots growth parameters, bioactive steviol glycosides (rebaudioside A and stevioside), and nonenzymatic antioxidant activities (total phenolic content (TPC), total flavonoid content (TFC), total antioxidant capacity (TAC), total reducing power (TRP) and 1,1-diphenyl-2-picrylhydrazyl(DPPH)-free radical scavenging activity (FRSA)) was elucidated. The significantly highest yield (92.4% direct shoot organogenesis) and secondary metabolites (2.94% Reb A, 2.52% ST, 95.3% DPPH-FRSA, 15.0% TPC, 13.0 µg/mg TFC, 22.3 µg/mg TAC, and 19.8 µg/mg TRP) production in response to abiotic stress elicitors was obtained in Murashige and Skoog (MS) medium treatment provided with 4% of PEG 6000. The overall trend was significant enhancement of growth dynamics and pharmaceutical compounds from control to 4% of PEG 6000 concentration as a defensive response against reactive oxygen species (ROS) produced in excess by water deficit. This is a very promising study to be employed in bioreactors to get markedly enhanced content of compounds of medicinal importance in the pharmaceutical market.

【 授权许可】

Unknown