Effect of Silk Fibroin on Cell Viability in Electrospun Scaffolds of Polyethylene Oxide

Polymers

Rafael Baltiérrez-Hoyos¹  VerónicaR. Vásquez-Garzón¹  Eduard De la Cruz-Burelo²  AnaíA. Valencia-Lazcano²  ManuelA. Valdés-Madrigal³  Gabriela Carrasco-Torres³  Ramón Román-Doval⁴ 

[1] CONACYT-Facultad de Medicina y Cirugía Universidad Autónoma Benito Juárez de Oaxaca, Ex Hacienda de Aguilera S/N, Carretera a San Felipe del Agua S/N, Oaxaca 68020, Mexico;Centro de Investigación y de Estudios Avanzados del IPN, Av. IPN 2508, la laguna Ticomán, Ciudad de México 07360, Mexico;Departamento de Nanociencias y Nanotecnología. Centro de Investigación y de Estudios Avanzados del IPN. Av. IPN 2508, la laguna Ticomán, Ciudad de México 07360, Mexico;Departamento de investigación y posgrado en alimentos, Facultad de Química, Universidad Autónoma de Querétaro, Centro universitario, Santiago de Querétaro, Querétaro 76010, Mexico;

关键词: silk fibroin;    surface modification;    electrospinning;    ultrafiltration;   

DOI  :  10.3390/polym11030451

来源: DOAJ

【 摘 要 】

In this study, a coating from electrospun silk fibroin was performed with the aim to modify the surface of breast implants. We evaluated the effect of fibroin on polymeric matrices of poly (ethylene oxide) (PEO) to enhance cell viability, adhesion, and proliferation of HaCaT human keratinocytes to enhance the healing process on breast prosthesis implantation. We electrospun six blends of fibroin and PEO at different concentrations. These scaffolds were characterized by scanning electron microscopy, contact angle measurements, ATR-FTIR spectroscopy, and X-ray diffraction. We obtained diverse network conformations at different combinations to examine the regulation of cell adhesion and proliferation by modifying the microstructure of the matrix to be applied as a potential scaffold for coating breast implants. The key contribution of this work is the solution it provides to enhance the healing process on prosthesis implantation considering that the use of these PEO–fibroin scaffolds reduced (p < 0.05) the amount of pyknotic nuclei. Therefore, viability of HaCaT human keratinocytes on PEO–fibroin matrices was significantly improved (p < 0.001). These findings provide a rational strategy to coat breast implants improving biocompatibility.

【 授权许可】

Unknown