Midbody Remnant Inheritance Is Regulated by the ESCRT Subunit CHMP4C

iScience

Leticia Labat-de-Hoz¹  Jaime Fernández-Barrera²  Miguel Bernabé-Rubio³  Armando Rubio-Ramos³  Miguel A. Alonso³  David G. Miguez³  Isabel Correas³  Javier Casares-Arias³  Juan Martín-Serrano³  Laura Rangel³  Leandro N. Ventimiglia⁴  Jessica B.A. Sadler⁴  María Ujué González⁵  Alvaro San Paulo⁵ 

[1] Department of Condensed Matter Physics, Universidad Autónoma de Madrid, 28049 Madrid, Spain;Department of Molecular Biology, Universidad Autónoma de Madrid, 28049 Madrid, Spain;Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, 28049 Madrid, Spain;Department of Infectious Diseases, Faculty of Life Sciences and Medicine, King's College London, London SE1 9RT, UK;Instituto de Micro y Nanotecnología, Consejo Superior de Investigaciones Científicas, 28760 Madrid, Spain;

关键词: Immunology;    Neuroscience;    Cell Biology;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: The inheritance of the midbody remnant (MBR) breaks the symmetry of the two daughter cells, with functional consequences for lumen and primary cilium formation by polarized epithelial cells, and also for development and differentiation. However, despite its importance, neither the relationship between the plasma membrane and the inherited MBR nor the mechanism of MBR inheritance is well known. Here, the analysis by correlative light and ultra-high-resolution scanning electron microscopy reveals a membranous stalk that physically connects the MBR to the apical membrane of epithelial cells. The stalk, which derives from the uncleaved side of the midbody, concentrates the ESCRT machinery. The ESCRT CHMP4C subunit enables MBR inheritance, and its depletion dramatically reduces the percentage of ciliated cells. We demonstrate (1) that MBRs are physically connected to the plasma membrane, (2) how CHMP4C helps maintain the integrity of the connection, and (3) the functional importance of the connection.

【 授权许可】

Unknown