Fetal acidosis in a sheep model: can we perform a second day of protocol in order to reduce the numbers of animals needed?

Clinical and Experimental Obstetrics & Gynecology

V. Houfflin-Debarge¹  L. Ghesquiere¹  P. Deruelle¹  C. Garabedian¹  J. De Jonckheere¹  E. Aubry¹  D. Sharma¹  C. Pierre¹  L. Storme¹ 

[1] University of Lille, EA 4489 – Perinatal Environment and Health, Lille, France;

关键词: fetal sheep;    animal experimentation;    animal ethics;    3r;    umbilical cord occlusion;    fetal acidosis;   

DOI  :  10.31083/j.ceog.2020.01.5120

来源: DOAJ

【 摘 要 】

Objective: To determine if a model of acidosis caused by occlusion of the umbilical cord could be repeated in the same sheep in ethicaly order to reduce the number of animals needed. Method: To obtain fetal acidosis, 1 min of total umbilical cord occlusion (UCO) every 2.5 min was performed on fetal sheep (n=7) until pH reached 7.10. Chronic instrumentation regularly recorded arterial blood gas and hemodynamic parameters. The occlusions were repeated to compare the data between the 2 days for each animal. Results: At the end of the UCO period, fetal acidosis was obtained with a pH 7.14 on day 1 (D1) and 7.06 on day 2 (D2). At baseline, fetuses were more hypotensive at D2 (41 mmHg vs 44 mmHg on D1, p < 0.05). During the UCO period, there was no significant difference in blood gas and hemodynamic data between D1 and D2. Conclusion: To reduce the number of animals needed, performing a second experiment on the same animal appears possible even if results must be interpreted cautiously when severe acidosis is reached.

【 授权许可】

Unknown