Clinical and Experimental Obstetrics & Gynecology | |
Fetal acidosis in a sheep model: can we perform a second day of protocol in order to reduce the numbers of animals needed? | |
V. Houfflin-Debarge1  L. Ghesquiere1  P. Deruelle1  C. Garabedian1  J. De Jonckheere1  E. Aubry1  D. Sharma1  C. Pierre1  L. Storme1  | |
[1] University of Lille, EA 4489 – Perinatal Environment and Health, Lille, France; | |
关键词: fetal sheep; animal experimentation; animal ethics; 3r; umbilical cord occlusion; fetal acidosis; | |
DOI : 10.31083/j.ceog.2020.01.5120 | |
来源: DOAJ |
【 摘 要 】
Objective: To determine if a model of acidosis caused by occlusion of the umbilical cord could be repeated in the same sheep in ethicaly order to reduce the number of animals needed. Method: To obtain fetal acidosis, 1 min of total umbilical cord occlusion (UCO) every 2.5 min was performed on fetal sheep (n=7) until pH reached 7.10. Chronic instrumentation regularly recorded arterial blood gas and hemodynamic parameters. The occlusions were repeated to compare the data between the 2 days for each animal. Results: At the end of the UCO period, fetal acidosis was obtained with a pH 7.14 on day 1 (D1) and 7.06 on day 2 (D2). At baseline, fetuses were more hypotensive at D2 (41 mmHg vs 44 mmHg on D1, p < 0.05). During the UCO period, there was no significant difference in blood gas and hemodynamic data between D1 and D2. Conclusion: To reduce the number of animals needed, performing a second experiment on the same animal appears possible even if results must be interpreted cautiously when severe acidosis is reached.
【 授权许可】
Unknown