Exerting the Appropriate Application of Methylprednisolone in Acute Spinal Cord Injury Based on Time Course Transcriptomics Analysis

International Journal of Molecular Sciences

Chang-Tze Ricky Yu¹  Liang-Yo Yang²  Shu-Hui Juan³  Yuan-Chii Gladys Lee⁴  Meng-Yu Tsai⁴  Hendrick Gao-Min Lim⁴  Shwu-Fen Chang⁵  Kory R. Johnson⁶  Yang C. Fann⁶  Jung-Chun Lin⁷ 

[1] Department of Applied Chemistry, National Chi Nan University, Nantou County 545, Taiwan;Department of Physiology, School of Medicine, China Medical University, Taichung 404, Taiwan;Department of Physiology, School of Medicine, Taipei Medical University, Taipei 110, Taiwan;Graduate Institute of Biomedical Informatics, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan;Graduate Institute of Medical Science, College of Medicine, Taipei Medical University, Taipei 110, Taiwan;Intramural IT and Bioinformatics Program, Division of Intramural, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA;School of Medical Laboratory and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan;

关键词: spinal cord injury;    methylprednisolone;    time course transcriptomics;    inflammation;    glycolysis;    oxidative stress;   

DOI  :  10.3390/ijms222313024

来源: DOAJ

【 摘 要 】

Methylprednisolone (MP) is an anti-inflammatory drug approved for the treatment of acute spinal cord injuries (SCIs). However, MP administration for SCIs has become a controversial issue while the molecular effects of MP remain unexplored to date. Therefore, delineating the benefits and side effects of MP and determining what MP cannot cure in SCIs at the molecular level are urgent issues. Here, genomic profiles of the spinal cord in rats with and without injury insults, and those with and without MP treatment, were generated at 0, 2, 4, 6, 8, 12, 24, and 48 h post-injury. A comprehensive analysis was applied to obtain three distinct classes: side effect of MP (SEMP), competence of MP (CPMP), and incapability of MP (ICMP). Functional analysis using these genes suggested that MP exerts its greatest effect at 8~12 h, and the CPMP was reflected in the immune response, while SEMP suggested aspects of metabolism, such as glycolysis, and ICMP was on neurological system processes in acute SCIs. For the first time, we are able to precisely reveal responsive functions of MP in SCIs at the molecular level and provide useful solutions to avoid complications of MP in SCIs before better therapeutic drugs are available.

【 授权许可】

Unknown