Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO2

Microbial Cell Factories

Junsong Wang¹  Zaiqiang Wu¹  Changhao Bi²  Yan Wang²  Jun Liu²  Xueli Zhang² 

[1] Center for Molecular Metabolism, School of Environmental and Biological Engineering, Nanjing University of Science and Technology;Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences;

关键词: Microbial electrosynthesis;    Bioelectrochemical systems;    Succinate;    CO2 fixation;   

DOI  :  10.1186/s12934-019-1067-3

来源: DOAJ

【 摘 要 】

Abstract Background Electrochemical energy is a key factor of biosynthesis, and is necessary for the reduction or assimilation of substrates such as CO2. Previous microbial electrosynthesis (MES) research mainly utilized naturally electroactive microbes to generate non-specific products. Results In this research, an electroactive succinate-producing cell factory was engineered in E. coli T110(pMtrABC, pFccA-CymA) by expressing mtrABC, fccA and cymA from Shewanella oneidensis MR-1, which can utilize electricity to reduce fumarate. The electroactive T110 strain was further improved by incorporating a carbon concentration mechanism (CCM). This strain was fermented in an MES system with neutral red as the electron carrier and supplemented with HCO3 +, which produced a succinate yield of 1.10 mol/mol glucose—a 1.6-fold improvement over the parent strain T110. Conclusions The strain T110(pMtrABC, pFccA-CymA, pBTCA) is to our best knowledge the first electroactive microbial cell factory engineered to directly utilize electricity for the production of a specific product. Due to the versatility of the E. coli platform, this pioneering research opens the possibility of engineering various other cell factories to utilize electricity for bioproduction.

【 授权许可】

Unknown