Nifedipine Protects INS-1 β-Cell from High Glucose-Induced ER Stress and Apoptosis

International Journal of Molecular Sciences

关键词: nifedipine;    Ca2+ homeostasis;    β-cell;    endoplasmic reticulum stress;    apoptosis;    high glucose;   

DOI  :  10.3390/ijms12117569

来源: DOAJ

【 摘 要 】

Sustained high concentration of glucose has been verified toxic to β-cells. Glucose augments Ca2+-stimulated insulin release in pancreatic β-cells, but chronic high concentration of glucose could induce a sustained level of Ca2+ in β-cells, which leads to cell apoptosis. However, the mechanism of high glucose-induced β-cell apoptosis remains unclear. In this study, we use a calcium channel blocker, nifedipine, to investigate whether the inhibition of intracellular Ca2+ concentration could protect β-cells from chronic high glucose-induced apoptosis. It was found that in a concentration of 33.3 mM, chronic stimulation of glucose could induce INS-1 β-cells apoptosis at least through the endoplasmic reticulum stress pathway and 10 μM nifedipine inhibited Ca2+ release to protect β-cells from high glucose-induced endoplasmic reticulum stress and apoptosis. These results indicated that inhibition of Ca2+ over-accumulation might provide benefit to attenuate islet β-cell decompensation in a high glucose environment.

【 授权许可】

Unknown