Lactobacillus rhamnosus GKLC1 ameliorates cisplatin-induced chronic nephrotoxicity by inhibiting cell inflammation and apoptosis

Biomedicine & Pharmacotherapy

Guan-Jhong Huang¹  Yen-Po Chen²  Yen-Lien Chen²  You-Shan Tsai²  Shih-Wei Lin³  Chin-Chu Chen⁴ 

[1] Department of Food Science, Nutrition and Nutraceutical Biotechnology, Shih Chien University, Taipei City 104336, Taiwan;Biotech Research Institute, Grape King Bio Ltd., Taoyuan City 325002, Taiwan;Department of Food Science and Biotechnology, National Chung Hsing University, Taichung City 402204, Taiwan;Institute of Food Science and Technology, National Taiwan University, Taipei City 106319, Taiwan;

关键词: Chemotherapeutic drug;    Chronic kidney disease (CKD);    Gut-kidney axis;    Inflammatory;    Oxidative stress;    Probiotics;   

DOI  :  

来源: DOAJ

【 摘 要 】

Sustained usage of the chemotherapeutic drug cisplatin may lead to chronic kidney disease (CKD). Despite cisplatin being toxic to the kidneys, the efficiency of its therapeutic effects cannot be completely replaced with other drugs. Probiotics can produce various strain-specific health-promoting effects and suppress many specific diseases. In this study, we present the alleviation of cisplatin-induced CKD with a probiotic, Lactobacillus rhamnosus GKLC1. Intermittent low doses of cisplatin were given to male CB57BL/6 mice (n = 6), which induced CKD symptoms such as weight loss, lesions in kidney tissue, and increases in blood urea nitrogen (BUN) and creatinine (CRE) in serum. The rats received two weeks of L. rhamnosus GKLC1 orally at doses of 125, 250, and 500 mg/kg B.W./day. After the treatment, significant dose-dependent reductions were observed in the kidney index, histopathological scoring, serum BUN, and CRE. An LLC-PK1 kidney cell assay revealed that L. rhamnosus GKLC1 suppressed the nephrotoxicity of cisplatin by reducing the inflammation via the MAPKs/NF-ĸB/COX-2 pathway, inhibiting apoptosis via the p53/Bax/Caspase-3 pathway, and ameliorating fibrosis via the STAT3 pathway. We conclude that L. rhamnosus GKLC1 could be applied as an agent to ameliorate the development of CKD.

【 授权许可】

Unknown