Accurate Detection of Rifampicin-Resistant Mycobacterium Tuberculosis Strains

Sensors

Keum-Soo Song¹  Satish Balasaheb Nimse²  Taisun Kim²  Jeongseong Yang³  Hee Jin Kim³ 

[1] Biometrix Technology, Inc. 202 BioVenture Plaza, Chuncheon 200-161, Korea;Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 200-702, Korea;Korea Mycobacterium Resource Center (KMRC), The Korean Institute of Tuberculosis, Cheongju-si 28158, Korea;

关键词: tuberculosis;    drug-resistant tuberculosis;    diagnosis;    accuracy;    sensitivity;    specificity;   

DOI  :  10.3390/s16030376

来源: DOAJ

【 摘 要 】

In 2013 alone, the death rate among the 9.0 million people infected with Mycobacterium tuberculosis (TB) worldwide was around 14%, which is unacceptably high. An empiric treatment of patients infected with TB or drug-resistant Mycobacterium tuberculosis (MDR-TB) strain can also result in the spread of MDR-TB. The diagnostic tools which are rapid, reliable, and have simple experimental protocols can significantly help in decreasing the prevalence rate of MDR-TB strain. We report the evaluation of the 9G technology based 9G DNAChips that allow accurate detection and discrimination of TB and MDR-TB-RIF. One hundred and thirteen known cultured samples were used to evaluate the ability of 9G DNAChip in the detection and discrimination of TB and MDR-TB-RIF strains. Hybridization of immobilized probes with the PCR products of TB and MDR-TB-RIF strains allow their detection and discrimination. The accuracy of 9G DNAChip was determined by comparing its results with sequencing analysis and drug susceptibility testing. Sequencing analysis showed 100% agreement with the results of 9G DNAChip. The 9G DNAChip showed very high sensitivity (95.4%) and specificity (100%).

【 授权许可】

Unknown