Sperm Behavior and Response to Melatonin under Capacitating Conditions in Three Sheep Breeds Subject to the Equatorial Photoperiod

Animals

Henry Alberto Grajales-Lombana¹  Adriana Casao²  Melissa Carvajal-Serna²  Rosaura Pérez-Pe²  Jaime Antonio Cardozo-Cerquera³ 

[1] Departamento de Producción Animal, Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Bogotá 111321, Colombia;Grupo BIOFITER (Biología, Fisiología y Tecnologías de la Reproducción), Departamento de Bioquímica y Biología Molecular y Celular, Instituto Universitario de Investigación en Ciencias Ambientales de Aragón (IUCA), Facultad de Veterinaria, Universidad de Zaragoza, 50013 Zaragoza, Spain;Researcher Center Tibaitatá, AGROSAVIA (Corporación Colombiana de Investigación Agropecuaria), Bogotá 250047, Colombia;

关键词: ram sperm;    equatorial;    photoperiod;    capacitation and melatonin;   

DOI  :  10.3390/ani11061828

来源: DOAJ

【 摘 要 】

In this study, we demonstrated that, in seasonal Mediterranean ovine breeds, supplementing the TALP medium with cAMP-elevating agents (the cocktail medium) is effective for achieving ram sperm capacitation, and that melatonin is able to regulate this phenomenon. We investigated the behavior under capacitating conditions using the TALP and cocktail mediums, and the response to melatonin, of spermatozoa from three sheep breeds (Colombian Creole, Romney Marsh, and Hampshire) subject to the equatorial photoperiod, during the dry and the rainy seasons. The cocktail medium was able to induce sperm capacitation, assayed by chlortetracycline staining and phosphotyrosine levels, to a greater extent than TALP, without a higher loss of viability (membrane integrity and viable spermatozoa without phosphatidylserine (PS) translocation). The addition of melatonin at 100 pM or 1 µM in the cocktail medium partially prevented the decrease in viability without PS translocation and the increase in capacitated spermatozoa from all breeds, with no significant effect on phosphotyrosine levels. Differences between breeds and seasons were evidenced. This study shows that melatonin is able to exert direct effects on spermatozoa in ovine breeds under equatorial photoperiod conditions, as it does in seasonal breeds located in temperate regions.

【 授权许可】

Unknown