Fusion of Chitin-Binding Domain From Chitinolyticbacter meiyuanensis SYBC-H1 to the Leaf-Branch Compost Cutinase for Enhanced PET Hydrolysis

Frontiers in Bioengineering and Biotechnology

Ren Wei¹  Zhongli Cui²  Rui Xue³  Yinping Chen³  Jie Zhou³  Min Jiang³  Huan Rong³  Weiliang Dong³ 

[1] Junior Research Group Plastic Biodegradation, Department of Biotechnology and Enzyme Catalysis, Institute of Biochemistry, University of Greifswald, Greifswald, Germany;Key Laboratory of Agricultural Environmental Microbiology, College of Life Science, Nanjing Agriculture University, Nanjing, China;State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China;

关键词: chitin-binding domain;    polyethylene terephthalate;    hydrolysis;    leaf-branch compost;    hydrophobicity;   

DOI  :  10.3389/fbioe.2021.762854

来源: DOAJ

【 摘 要 】

Polyethylene terephthalate (PET) is a mass-produced petroleum-based non-biodegradable plastic that contributes to the global plastic pollution. Recently, biocatalytic degradation has emerged as a viable recycling approach for PET waste, especially with thermophilic polyester hydrolases such as a cutinase (LCC) isolated from a leaf-branch compost metagenome and its variants. To improve the enzymatic PET hydrolysis performance, we fused a chitin-binding domain (ChBD) from Chitinolyticbacter meiyuanensis SYBC-H1 to the C-terminus of the previously reported LCCICCG variant, demonstrating higher adsorption to PET substrates and, as a result, improved degradation performance by up to 19.6% compared to with its precursor enzyme without the binding module. For compare hydrolysis with different binding module, the catalytic activity of LCCICCG-ChBD, LCCICCG-CBM, LCCICCG-PBM and LCCICCG-HFB4 were further investigated with PET substrates of various crystallinity and it showed measurable activity on high crystalline PET with 40% crystallinity. These results indicated that fusing a polymer-binding module to LCCICCG is a promising method stimulating the enzymatic hydrolysis of PET.

【 授权许可】

Unknown