Taxonomic and Metabolite Diversities of Moss-Associated Actinobacteria from Thailand

Metabolites

Naowarat Cheeptham¹  Jianping Xu²  Chadabhorn Insuk²  Adrian Forsythe²  Satoshi Ōmura³  Atsuko Matsumoto³  Pornkanok Pongpamorn⁴  Wasu Pathom-aree⁵ 

[1] Department of Biological Sciences, Faculty of Science, Thompson Rivers University, Kamloops, BC V2C 0C8, Canada;Department of Biology, McMaster University, Hamilton, ON L8S 4K1, Canada;Kitasato Institute for Life Sciences, Kitasato University, Minato-ku, Tokyo 108-8641, Japan;National Omics Center, National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand;Research Center of Microbial Diversity and Sustainable Utilization, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand;

关键词: actinobacteria;    moss;    selective isolation;    plant growth promotion;    MRSA (methicillin-resistant Staphylococcus aureus);    white-nose syndrome;   

DOI  :  10.3390/metabo12010022

来源: DOAJ

【 摘 要 】

Actinobacteria are a group of ecologically important bacteria capable of producing diverse bioactive compounds. However, much remains unknown about the taxonomic and metabolic diversities of actinobacteria from many geographic regions and ecological niches. In this study, we report the isolation of actinobacteria from moss and moss-associated rhizosphere soils in Thailand. Among the 89 isolates analyzed for their bioactivities, 86 strains produced indole-3-acetic acid (IAA, ranging from 0.04 to 59.12 mg/L); 42 strains produced hydroxamate type of siderophore; 35 strains produced catecholate type of siderophore; 21 strains solubilized tricalcium phosphate; and many strains exhibited antagonistic activities against one to several of the seven selected plant, animal, and human pathogens. Overall, actinobacteria from the rhizosphere soil of mosses showed greater abilities to produce IAA and siderophores and to solubilize tricalcium phosphate than those from mosses. Among these 89 isolates, 37 were analyzed for their 16S rRNA gene sequences, which revealed their diverse phylogenetic distributions among seven genera, Streptomyces, Micromonospora, Nocardia, Actinoplanes, Saccharothrix, Streptosporangium, and Cryptosporangium. Furthermore, gas chromatography-mass spectrometry analyses of ethyl acetate crude extracts of three selected isolates with inhibitory effects against a methicillin-resistant Staphylococcus aureus strain revealed diverse metabolites with known antimicrobial activities. Together, our results demonstrate that actinobacteria from mosses in Thailand are taxonomically diverse and capable of producing a range of metabolites with plant-growth-promoting and microbial pathogen-inhibiting potentials.

【 授权许可】

Unknown