Effects of Cryopreservation on Cell Metabolic Activity and Function of Biofabricated Structures Laden With Osteoblasts

Materials

Jozef Kaiser¹  Edgar B. Montufar¹  Ladislav Čelko¹  Laura G. HernándezTapia¹  Jan Žídek¹  Zdenka Fohlerová¹  Marco A. AlvarezPerez² 

[1] CEITEC—Central European Institute of Technology, Brno University of Technology, Purkynova 123, 612 00 Brno, Czech Republic;Tissue Bioengineering Laboratory, Faculty of Dentistry, Universidad Nacional Autónoma de México, Coyoacan, 04510 Mexico City, Mexico;

关键词: biofabrication;    bioprinting;    cryopreservation;    bone construct;    osteoblast;    metabolic activity;   

DOI  :  10.3390/ma13081966

来源: DOAJ

【 摘 要 】

Biofabrication and maturation of bone constructs is a longterm task that requires a high degree of specialization. This specialization falls onto the hierarchy complexity of the bone tissue that limits the transfer of this technology to the clinic. This work studied the effects of the shortterm cryopreservation on biofabricated osteoblastcontaining structures, with the final aim to make them steadily available in biobanks. The biological responses studied include the osteoblast postthawing metabolic activity and the recovery of the osteoblastic function of 3Dbioprinted osteoblastic structures and beta tricalcium phosphate (βTCP) scaffolds infiltrated with osteoblasts encapsulated in a hydrogel. The obtained structures were cryopreserved at −80 C for 7 days using dimethyl sulfoxide (DMSO) as cryoprotectant additive. After thawing the structures were cultured up to 14 days. The results revealed fundamental biological aspects for the successful cryopreservation of osteoblast constructs. In summary, immature osteoblasts take longer to recover than mature osteoblasts. The precryopreservation culture period had an important effect on the metabolic activity and function maintain, faster recovering normal values when cryopreserved after longerterm culture (7 days). The use of βTCP scaffolds further improved the osteoblast survival after cryopreservation, resulting in similar levels of alkaline phosphatase activity in comparison with the nonpreserved structures. These results contribute to the understanding of the biology of cryopreserved osteoblast constructs, approaching biofabrication to the clinical practice.

【 授权许可】

Unknown