期刊论文详细信息
Frontiers in Veterinary Science
Inhibitory Effect of Bovine Adipose-Derived Mesenchymal Stem Cells on Lipopolysaccharide Induced Inflammation of Endometrial Epithelial Cells in Dairy Cows
Jidong Jin1  Dongbo Sun2  Shixin Fu2  Wengeng Lu2  Ying-Hao Han3  Ying-Ying Mao3  Jia-Bin Yu3  Wei-Long Li3  Nan-Nan Yu3  Zhenzhen Du4  Qing Liu4  Linqing Si4  Zheng-Mei Xu4  Siqi Yuan4 
[1] Cofeed Feedmill (Changchun) Co., Ltd., Changchun, China;Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, China;Laboratory of Stem Cell Therapy and Regenration Biology, College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing, China;Laboratory of Theriogenology and Reproductive Health, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, China;
关键词: bovine adipose-derived mesenchymal stem cell;    inflammation;    lipopolysaccharide;    endometritis;    dairy cows;   
DOI  :  10.3389/fvets.2021.726328
来源: DOAJ
【 摘 要 】

Endometritis is a disease that affects reproductive health in dairy cows and causes serious economic damage to the dairy industry world-wide. Although in recent years, the application of mesenchymal stem cell (MSC) therapy for the treatment of inflammatory diseases has attracted much attention, there are few reports of the use of MSCs in dairy cows. In the present study, our objective was to explore the inhibitory effects of bovine adipose-derived mesenchymal stem cells (bAD-MSCs) on lipopolysaccharide (LPS) induced inflammation in bovine endometrial epithelial cells (bEECs) along with the potential underlying molecular mechanisms. We characterized isolated bAD-MSCs using cell surface marker staining and adipogenic/osteogenic differentiation, and analyzed them using immunofluorescence, flow cytometry (surface marker staining), and adipogenic and osteogenic differentiation. Furthermore, to understand the anti-inflammatory effects of bAD-MSCs on LPS induced bEEC inflammation, we used a bAD-MSC/bEEC co-culture system. The results showed that bAD-MSC treatments could significantly decrease LPS induced bEEC apoptosis and pro-inflammatory cytokine expression levels, such as interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α). Furthermore, our results showed that bAD-MSC treatments could also significantly downregulate LPS induced p38, IkB-a, and JAK1 phosphorylation and Bax protein expression levels, which are closely related to inflammatory progress and cellular apoptosis in bEECs. Our findings demonstrate that bAD-MSCs play an inhibitory role in LPS induced bEEC inflammation and provide new insights for the clinical therapy of endometritis in dairy cows.

【 授权许可】

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