Detection of Reactive Oxygen and Nitrogen Species by Electron Paramagnetic Resonance (EPR) Technique

Molecules

Sibel Suzen¹  Hande Gurer-Orhan²  Luciano Saso³ 

[1] Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Ankara University, Tandogan, Ankara 06100, Turkey;Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Ege University, 35100 Izmir, Turkey;Department of Physiology and Pharmacology “Vittorio Erspamer”, Sapienza University of Rome, P. le Aldo Moro 5, 00185 Rome, Italy;

关键词: free radical;    oxidative stress;    EPR;    ROS;    RNS;   

DOI  :  10.3390/molecules22010181

来源: DOAJ

【 摘 要 】

During the last decade there has been growing interest in physical-chemical oxidation processes and the behavior of free radicals in living systems. Radicals are known as intermediate species in a variety of biochemical reactions. Numerous techniques, assays and biomarkers have been used to measure reactive oxygen and nitrogen species (ROS and RNS), and to examine oxidative stress. However, many of these assays are not entirely satisfactory or are used inappropriately. The purpose of this chapter is to review current EPR (Electron Paramagnetic Resonance) spectroscopy methods for measuring ROS, RNS, and their secondary products, and to discuss the strengths and limitations of specific methodological approaches.

【 授权许可】

Unknown