Genetic Diversity and Distribution of Virulence-Associated Genes in Y. enterocolitica and Y. enterocolitica-Like Isolates from Humans and Animals in Poland

Pathogens

Maciej Kuczkowski¹  Anna Woźniak-Biel¹  Alina Wieliczko¹  Justyna Schubert²  Jarosław Bystroń²  Jacek Bania²  Katarzyna Morka²  Ewa Wałecka-Zacharska²  Gabriela Bugla-Płoskońska³  Bartłomiej Dudek³ 

[1] Department of Epizootiology and Clinic of Birds and Exotic Animals, Wrocław University of Environmental and Life Sciences, pl. Grunwaldzki 45, 50-366 Wrocław, Poland;Department of Food Hygiene and Consumer Health Protection, Wrocław University of Environmental and Life Sciences, C. K. Norwida 31, 50-375 Wrocław, Poland;Department of Microbiology, Institute of Genetics and Microbiology, Wroclaw University, S. Przybyszewskiego 63, 51-148 Wrocław, Poland;

关键词: virulence-associated genes;    wild boars;    Yersinia enterocolitica;   

DOI  :  10.3390/pathogens10010065

来源: DOAJ

【 摘 要 】

Yersinia enterocolitica, widespread within domestic and wild-living animals, is a foodborne pathogen causing yersiniosis. The goal of this study was to assess a genetic similarity of Y. enterocolitica and Y. enterocolitica-like strains isolated from different hosts using Multiple Locus Variable-Number Tandem Repeat Analysis (MLVA) and Pulsed-Field Gel Electrophoresis (PFGE) methods, and analyze the prevalence of virulence genes using multiplex-Polymerase Chain Reaction (PCR) assays. Among 51 Yersinia sp. strains 20 virulotypes were determined. The most common virulence genes were ymoA, ureC, inv, myfA, and yst. Yersinia sp. strains had genes which may contribute to the bacterial invasion and colonization of the intestines as well as survival in serum. One wild boar Y. enterocolitica 1A strain possessed ail gene implying the possible pathogenicity of 1A biotype. Wild boar strains, represented mainly by 1A biotype, were not classified into the predominant Variable-Number Tandem Repeats (VNTR)/PFGE profile and virulotype. There was a clustering tendency among VNTR/PFGE profiles of pig origin, 4/O:3, and virulence profile. Pig and human strains formed the most related group, characterized by ~80% of genetic similarity what suggest the role of pigs as a potential source of infection for the pork consumers.

【 授权许可】

Unknown