In Vitro Anticancer Activity and Structural Characterization of Ubiquinones from Antrodia cinnamomea Mycelium

Molecules

Kuen-Tze Lin¹  Feng-Yi Lai²  Shih-Yu Lee²  I-Chuan Yen³  Mao-Tien Kuo⁴  Wen-Liang Chang⁵ 

[1] Department of Radiation Oncology, Tri-Service General Hospital, National Defense Medical Center, Taipei 114, Taiwan;Graduate Institute of Aerospace and Undersea Medicine, National Defense Medical Center, Taipei 114, Taiwan;Graduate Institute of Medical Science, National Defense Medical Center, No.116, Sec.6, Minchuan East Road, Neihu District, Taipei 114, Taiwan;LanTyng Biotech, Co, Ltd., Taipei 114, Taiwan;School of Pharmacy, National Defense Medical Center, Taipei 114, Taiwan;

关键词: Antrodia cinnamomea;    ubiquinone derivative;    antrocinnamone;    4-acetylantrocamol LT3;    HPLC analysis and fingerprint;    cytotoxicity;    flow cytometry;    cell cycle;   

DOI  :  10.3390/molecules22050747

来源: DOAJ

【 摘 要 】

Two new ubiquinones, named antrocinnamone and 4-acetylantrocamol LT3, were isolated along with six known ubiquinones from Antrodia cinnamomea (Polyporaceae) mycelium. The developed HPLC analysis methods successfully identified eight different ubiquinones, two benzenoids, and one maleic acid derivative from A. cinnamomea. The ubiquinones 1–8 exhibited potential and selective cytotoxic activity against three human cancer cell lines, with IC50 values ranging from 0.001 to 35.883 μM. We suggest that the different cytotoxicity levels were related to their chemical structures, especially the 4-hydroxycyclohex-2-enone ring and the presence of a free hydroxyl group in the side chain. The suppression by 4-acetylantrocamol LT3 stopped the cell cycle at the beginning of the G2-M phase thus making the cell cycle arrest at the sub-G1 phase as compared with control cells.

【 授权许可】

Unknown