Combining rare alleles and grouped pollen donors to assign paternity in pollen dispersal studies

Applications in Plant Sciences

Vijay K. Nandula¹  Micheal D. K. Owen²  Berish Y. Rubin³  Sylvia L. Anderson³  Randolph G. Gardner⁴  Chelsea L. Butcher⁵  J. D. Lewis⁵ 

[1] Crop Production Systems Research Unit, Agricultural Research Service U.S. Department of Agriculture 141 Experiment Station Road Stoneville Mississippi38776USA;Department of Agronomy Iowa State University 716 Farm House Lane Ames Iowa50011USA;Department of Biological Sciences Fordham University 441 East Fordham Road Bronx New York10458USA;Department of Horticultural Science North Carolina State UniversityMountain Horticultural Crops Research and Extension Center (MHCREC) 455 Research Drive Mills River North Carolina28759USA;Louis Calder Center, Biological Field Station Fordham University 31 Whippoorwill Road Armonk New York10504USA;

关键词: Amaranthaceae;    experimental arrays;    gene flow;    paternity assignment;    pollen dispersal;    Solanaceae;   

DOI  :  10.1002/aps3.11330

来源: DOAJ

【 摘 要 】

Premise Pollen dispersal plays a critical role in gene flow of seed plants. Most often, pollen dispersal is measured using paternity assignment. However, this approach can be time‐consuming because it typically entails genotyping all pollen donors, receptors, and offspring at several molecular markers. Methods We developed a faster, simpler protocol to track paternity, using pollen receptors and grouped pollen donors that possess rare alleles. We tested this approach using wind‐pollinated Amaranthus tuberculatus and insect‐pollinated Solanum lycopersicum. After screening potential markers for rare alleles, we grew both species in experimental arrays under field conditions. Results All tested A. tuberculatus seeds and 97% of S. lycopersicum fruits could be assigned to the grouped pollen donors using each of two markers. From these results, we could infer paternity of untested offspring and assess pollen dispersal patterns in each array. Discussion By combining rare alleles and grouped pollen donors, we could assess pollen dispersal for both species and across all arrays after genotyping a small number of pollen donors and a representative subset of offspring. While directly applicable to A. tuberculatus and S. lycopersicum, this approach could be used in other species to assess pollen dispersal under field conditions.

【 授权许可】

Unknown