Genetic and Phenotypic Characterization of the Etiological Agent of Canine Orchiepididymitis Smooth Brucella sp. BCCN84.3

Frontiers in Veterinary Science

Elena Campos¹  Caterina Guzmán-Verri²  Carlos Chacón-Díaz²  Raquel Conde-Álvarez³  Beatriz Aragón-Aranda³  Ignacio Moriyón³  Michel S. Zygmunt⁴  Mathieu Gonnet⁴  Axel Cloeckaert⁴  Kate S. Baker⁵  Nicholas R. Thomson⁶  Edgardo Moreno⁷  Nazareth Ruíz-Villalobos⁷  Eunice Víquez-Ruiz⁷  Marcela Suárez-Esquivel⁷  Pilar M. Muñoz⁸  José María Blasco⁸ 

[1] Centro Nacional de Referencia en Bacteriología, Instituto Costarricense de Investigación y Enseñanza en Nutrición y Salud (INCIENSA), Cartago, Costa Rica;Facultad de Microbiología, Centro de Investigación en Enfermedades Tropicales, Universidad de Costa Rica, San José, Costa Rica;IDISNA and Departamento de Microbiología y Parasitología, Instituto de Salud Tropical, Universidad de Navarra, Pamplona, Spain;ISP, INRA, Université François Rabelais de Tours, Nouzilly, France;Institute for Integrative Biology, University of Liverpool, Liverpool, United Kingdom;Pathogen Genomics, Wellcome Trust Sanger Institute, Hinxton, United Kingdom;Programa de Investigación en Enfermedades Tropicales (PIET), Escuela de Medicina Veterinaria, Universidad Nacional, Heredia, Costa Rica;Unidad de Producción y Sanidad Animal, Instituto Agroalimentario de Aragón-IA2, CITA-Universidad de Zaragoza, Zaragoza, Spain;

关键词: Brucella;    Brucella melitensis;    Brucella suis;    Brucella canis;    brucellosis;    dog;   

DOI  :  10.3389/fvets.2019.00175

来源: DOAJ

【 摘 要 】

Members of the genus Brucella cluster in two phylogenetic groups: classical and non-classical species. The former group is composed of Brucella species that cause disease in mammals, including humans. A Brucella species, labeled as Brucella sp. BCCN84.3, was isolated from the testes of a Saint Bernard dog suffering orchiepididymitis, in Costa Rica. Following standard microbiological methods, the bacterium was first defined as “Brucella melitensis biovar 2.” Further molecular typing, identified the strain as an atypical “Brucella suis.” Distinctive Brucella sp. BCCN84.3 markers, absent in other Brucella species and strains, were revealed by fatty acid methyl ester analysis, high resolution melting PCR and omp25 and omp2a/omp2b gene diversity. Analysis of multiple loci variable number of tandem repeats and whole genome sequencing demonstrated that this isolate was different from the currently described Brucella species. The smooth Brucella sp. BCCN84.3 clusters together with the classical Brucella clade and displays all the genes required for virulence. Brucella sp. BCCN84.3 is a species nova taxonomical entity displaying pathogenicity; therefore, relevant for differential diagnoses in the context of brucellosis. Considering the debate on the Brucella species concept, there is a need to describe the extant taxonomical entities of these pathogens in order to understand the dispersion and evolution.

【 授权许可】

Unknown