Transcriptional Regulation of PIK3CD and PIKFYVE in T-Cell Acute Lymphoblastic Leukemia by IKAROS and Protein Kinase CK2

International Journal of Molecular Sciences

Tommy Hu¹  MohammadAtiqur Rahman¹  Daniel Bogush¹  Chandrika Gowda¹  Morgann Klink¹  Elanora Dovat¹  Yali Ding¹  Mario Soliman¹  Shriya Kane¹  PavanKumar Dhanyamraju¹  Mary McGrath¹  Chunhua Song¹  Arati Sharma²  Dhimant Desai² 

[1] Department of Pediatrics, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA;Department of Pharmacology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA;

关键词: IKAROS;    protein kinase CK2;    T cell acute lymphoblastic leukemia;    PI3K;    transcriptional regulation;   

DOI  :  10.3390/ijms22020819

来源: DOAJ

【 摘 要 】

IKAROS, encoded by the IKZF1 gene, is a DNA-binding protein that functions as a tumor suppressor in T cell acute lymphoblastic leukemia (T-ALL). Recent studies have identified IKAROS’s novel function in the epigenetic regulation of gene expression in T-ALL and uncovered many genes that are likely to be directly regulated by IKAROS. Here, we report the transcriptional regulation of two genes, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta (PIK3CD) and phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), by IKAROS in T-ALL. PIK3CD encodes the protein p110δ subunit of phosphoinositide 3-kinase (PI3K). The PI3K/AKT pathway is frequently dysregulated in cancers, including T-ALL. IKAROS binds to the promoter regions of PIK3CD and PIKFYVE and reduces their transcription in primary T-ALL. Functional analysis demonstrates that IKAROS functions as a transcriptional repressor of both PIK3CD and PIKFYVE. Protein kinase CK2 (CK2) is a pro-oncogenic kinase that is overexpressed in T-ALL. CK2 phosphorylates IKAROS, impairs IKAROS’s DNA-binding ability, and functions as a repressor of PIK3CD and PIKFYVE. CK2 inhibition results in increased IKAROS binding to the promoters of PIK3CD and PIKFYVE and the transcriptional repression of both these genes. Overall, the presented data demonstrate for the first time that in T-ALL, CK2 hyperactivity contributes to PI3K signaling pathway upregulation, at least in part, through impaired IKAROS transcriptional regulation of PIK3CD and PIKFYVE. Targeting CK2 restores IKAROS’s regulatory effects on the PI3K oncogenic signaling pathway.

【 授权许可】

Unknown