A 3D Bioprinted Human Meniscus Shape Enriched with Mesenchymal Cells

Applied Sciences

Mihai Ionac¹  Carmen Tatu²  Sergiu Galatanu³  Daniela Puscasiu⁴  Catalin Adrian Miu⁵  Fabian Tatu⁵  Mihai Hurmuz⁵ 

[1] 2nd Department of Surgery, “Victor Babes” University of Medicine and Pharmacy Timisoara, 2A Eftimie Murgu Sq., 300041 Timisoara, Romania;Department of Functional Sciences, “Victor Babes” University of Medicine and Pharmacy Timisoara, 2A Eftimie Murgu Sq., 300041 Timisoara, Romania;Department of Mechanics and Strength of Materials, University Politehnica Timișoara, 1 Mihai Viteazul Blvd., 300222 Timisoara, Romania;Department of Microscopic Morphology, “Victor Babes” University of Medicine and Pharmacy Timisoara, 2A Eftimie Murgu Sq., 300041 Timisoara, Romania;Department of Orthopaedics, Urology and Medical Imaging, “Victor Babes” University of Medicine and Pharmacy Timisoara, 2A.Eftimie Murgu Sq., 300041 Timisoara, Romania;

关键词: bioprinting;    biomeniscus;    hydrogel;    regenerative medicine;   

DOI  :  10.3390/app112411733

来源: DOAJ

【 摘 要 】

Background and objectives: Regenerative medicine, with its massive development over the years, has the potential to solve some of the most problematic medical issues, such as functional organ transplantation. The aim of this study was to create a human meniscal shape 3D-printed enriched with human adipose-derived mesenchymal cells. Materials and Methods: Human infrapatellar fat pad was harvested, and mesenchymal cells were isolated. The mesenchymal stem cells were differentiated to the chondrocite lineage and a hydrogel (a nanofibrillar cellulose, sodium alginate, D-mannitol, and Hepes buffer solution combination) cell mixture was bioprinted to create three human-size meniscus structures. The obtained structures were evaluated regarding the cell viability, appropriate size in relation to a native meniscus, and some mechanical characteristics. Results: The human meniscal shape created respected the anatomic characteristic of a native structure. Cell viability of approximately 97% and extracellular matrix formation after the printing process were observed. The mean maximum force for the meniscus with mesenchymal cells was 6.5 N (+/−0.5 N) compared to the mean maximum force for the native meniscus of 10.32 N (+/−0.7 N), which is statistically relevant (p < 0.01). Conclusion: This paper presents the potential of bioprinting viable cell structures that could in the future present enough mechanical strength to replace a human organ, such as a meniscus. There are still limitations regarding the ink and the printing process, but we are confident that these problems will soon be solvable.

【 授权许可】

Unknown