The application of selenium nanoparticles for enhancing the efficacy of photodynamic inactivation of planktonic communities and the biofilm of Streptococcus mutans

BMC Research Notes

Zahra Yadegari¹  Davood Darban-Sarokhalil²  Samane Shahmoradi²  Aref Shariati³  Seyed Mohammad Amini⁴  Nazanin Zargar⁵ 

[1] Department of Dental Biomaterials, Dental School, Shahid Beheshti University of Medical Sciences;Department of Microbiology, School of Medicine, Iran University of Medical Sciences;Molecular and Medicine Research Center, Khomein University of Medical Sciences;Radiation Biology Research Center, Iran University of Medical Sciences;School of Dentistry, Shahid Beheshti University of Medical Sciences;

关键词: Selenium nanoparticles;    Antimicrobial photodynamic therapy;    Combination therapy;    Biofilm;    S. mutans;   

DOI  :  10.1186/s13104-022-05973-w

来源: DOAJ

【 摘 要 】

Abstract Objective Streptococcus mutans is one of the principal causative agents of dental caries (tooth decay) found in the oral cavity. Therefore, this study investigates whether selenium nanoparticles (SeNPs) enhance the efficacy of photodynamic therapy (PDT) against both planktonic communities and the one-day-old biofilm of S. mutans. In this study, the planktonic and 24-h biofilm of S. mutans have been prepared in 96-cell microplates. These forms were treated by methylene blue (MB) and SeNPs and then were exposed to light-emitting diode (LED) lighting. Finally, the results have been reported as CFU/ml. Results The outcomes demonstrated that MB-induced PDT and SeNPs significantly reduced the number of planktonic bacteria (P-value < 0.001). The comparison between the treated and untreated groups showed that combining therapy with SeNPs and PDT remarkably decreased colony-forming units of one-day-old S. mutans biofilm (P-value < 0.05). The findings revealed that PDT modified by SeNPs had a high potential to destroy S. mutans biofilm. This combination therapy showed promising results to overcome oral infection in dental science.

【 授权许可】

Unknown