Identification and characterization of Aspergillus fumigatus isolates from broilers

Pesquisa Veterinária Brasileira

关键词: Aspergillus fumigatus;    broilers;    molecular identification;    multiplex PCR;    genotyping;   

DOI  :  10.1590/S0100-736X2016000700005

来源: DOAJ

【 摘 要 】

Abstract: Aspergillosis is one of the main causes of mortality in birds. The pulmonary system is most frequently affected, with lesions observed in the air sacs and lungs of a wide variety of bird species. The aim of this study was to confirm by molecular methods the identification and the genetic diversity of Aspergillus fumigatus isolates of lung's samples from healthy broilers (Galus galus domesticus). Forty-four (9.5%) isolates of lung's samples were confirmed as A. fumigatus by polymerase chain reaction (PCR) multiplex (amplification of β-tub and rodA gene fragments). Microsatellite typing for A. fumigatus was used to analyse all avian isolates. Among them, 40 genotypes (90.9%) were observed only one time. The results showed a high variability and multiple genotypes of de A. fumigatus collected from lung's samples of broilers.

【 授权许可】

Unknown